ArticleStem cells and development2011
Homologous recombination conserves DNA sequence integrity throughout the cell cycle in embryonic stem cells.
Article in Stem cells and development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Phosphorylation and DNA damage resolution coordinate SOX2-mediated reprogramming in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular Mechanisms of DNA Damage Response and Epigenetic Regulation in Cold-Adapted Species: Implications for Genome Stability and Molecular Network Perspective.Current issues in molecular biology · 2025Review
- Molecular mechanisms of extrachromosomal circular DNA formation.Nucleic acids research · 2025Review
- Cell-Type-Dependent Recruitment Dynamics of FUS Protein at Laser-Induced DNA Damage Sites.International journal of molecular sciences · 2024Article
- Tools used to assay genomic instability in cancers and cancer meiomitosis.Journal of cell communication and signaling · 2022Review
- Networks and Islands of Genome Nano-architecture and Their Potential Relevance for Radiation Biology : (A Hypothesis and Experimental Verification Hints).Results and problems in cell differentiation · 2022Article
- Regulation and coordination of the different DNA damage responses inFrontiers in cell and developmental biology · 2022Review
- Review
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- Stage-Specific Effects of Ionizing Radiation during Early Development.International journal of molecular sciences · 2020Review
- DNA repair fidelity in stem cell maintenance, health, and disease.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- IDH1-R132H acts as a tumor suppressor in glioma via epigenetic up-regulation of the DNA damage response.Science translational medicine · 2019Article
- Differentiation of Human Induced Pluripotent or Embryonic Stem Cells Decreases the DNA Damage Repair by Homologous Recombination.Stem cell reports · 2017Article
- Effect of ionizing radiation on the proliferation of human embryonic stem cells.Scientific reports · 2017Article
- Sirtuins and DNA damage repair: SIRT7 comes to play.Nucleus (Austin, Tex.) · 2017Review
- SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair.The EMBO journal · 2016Article
- Pluripotent Stem Cells and DNA Damage Response to Ionizing Radiations.Radiation research · 2016Review
- Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells.PLoS genetics · 2016Article
- Genetic stability of pluripotent stem cells during anti-cancer therapies.Experimental and therapeutic medicine · 2016Article
- Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
The maintenance of genomic integrity is crucial to embryonic stem cells (ESC) considering the potential for propagating undesirable mutations to the resulting somatic and germ cell lineages. Indeed, mouse ESC (mESC) exhibit a significantly lower mutation frequency compared to differentiated cells. This could be due to more effective elimination of genetically damaged cells via apoptosis, or especially robust, sequence-conserving DNA damage repair mechanisms such as homologous recombination (HR). We used fluorescence microscopy and 3-dimensional image analysis to compare mESC and differentiated cells, with regard to HR-mediated repair of spontaneous and X-ray-induced double-strand breaks (DSBs). Microscopic analysis of repair foci, flow cytometry, and functional assays of the major DSB repair pathways indicate that HR is greater in mESC compared to fibroblasts. Strikingly, HR appears to be the predominant pathway choice to repair induced or spontaneous DNA damage throughout the ESC cycle in contrast to fibroblasts, where it is restricted to replicated chromatin. This suggests that alternative templates, such as homologous chromosomes, are more frequently used to repair DSB in ESC. Relatively frequent HR utilizing homolog chromosome sequences preserves genome integrity in ESC and has distinctive and important genetic consequences to subsequent somatic and germ cell lineages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.