ArticleCurrent microbiology2011
Pseudomonas aeruginosa pvdQ gene prevents Caco-2 cells from obstruction of quorum-sensing signal.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Choosing an appropriate infection model to study quorum sensing inhibition in Pseudomonas infections.International journal of molecular sciences · 2013Review
- Influence of Pseudomonas aeruginosa pvdQ gene on altering antibiotic susceptibility under swarming conditions.Current microbiology · 2013Article
- Influence of Pseudomonas aeruginosa pvdQ gene on altering antibiotic susceptibility under swarming conditions.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quorum sensing (QS) system plays an important role in bacterial pathopoiesis of incurable Pseudomonas aeruginosa infection, which strongly warrants new strategies for absence of curative treatment to date. Latest investigations show that pvdQ gene of P. aeruginosa can attenuate the pathopoiesis of the bacteria by encoding acylase enzyme and hydrolyze N-(3-oxododecanoyl)-Homoserine Lactone (3O-oxo-C(12)-HSL), the key signal molecule of QS system. This study tries to resist the pathogenicity of P. aeruginosa by transfecting human intestinal epithelial Caco-2 cells with pvdQ gene. We found that 3O-oxo-C(12)-HSL was decreased in the supernatant of cells transfected with pvdQ gene. Moreover, the result of flow cytometry showed that the 3O-oxo-C(12)-HSL evoked apoptosis rate of Caco-2 cells was inhibited when the cells were transfected with pvdQ gene. In contrast, the control result displayed increased Caco-2 cells' apoptosis rate after stimulation of 3O-oxo-C(12)-HSL without protection of pvdQ gene. In conclusion, we successfully protect mammalian cells Caco-2 from injure of QS signal molecule 3O-oxo-C(12)-HSL through imputing pvdQ gene, which may suggest a new therapeutic strategy for P. aeruginosa infection.
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