ArticleProceedings of the National Academy of Sciences of the United States of America2010
A role for tetrahydrofolates in the metabolism of iron-sulfur clusters in all domains of life.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing.ACS synthetic biology · 2024Pooled it
- Genome-wide analysis of the Escherichia coli Keio collection reveals genetic determinants associated with ferulic acid responses.Folia microbiologica · 2026Article
- The iron-sulfur accelerator YgfZ modulates genome-wide IHF-binding dynamics to regulate replication initiation inFrontiers in microbiology · 2026Article
- Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.Plant direct · 2025Article
- Classification ofiScience · 2024Article
- Essential Oil ofPlants (Basel, Switzerland) · 2023Article
- Intermittent antibiotic treatment of bacterial biofilms favors the rapid evolution of resistance.Communications biology · 2023Article
- Essentiality of theInternational journal of molecular sciences · 2023Article
- Functional Prediction of Microbial Communities in Sediment Microbial Fuel Cells.Bioengineering (Basel, Switzerland) · 2023Article
- A novel IBA57 variant is associated with mitochondrial iron-sulfur protein deficiency and necrotizing myelopathy in dogs.Frontiers in genetics · 2023Article
- Article
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- A photoaffinity probe that targets folate-binding proteins.Bioorganic & medicinal chemistry letters · 2021Article
- MgrB Inactivation Confers Trimethoprim Resistance inFrontiers in microbiology · 2021Article
- One-carbon metabolism, folate, zinc and translation.Microbial biotechnology · 2020Article
- First Step in Catalysis of the RadicalJournal of the American Chemical Society · 2020Article
- Effects of Arsenic on Trichloroethene-Dechlorination Activities ofEnvironmental science & technology · 2020Article
- Foliar Spraying with Compound Amino Acid-Iron Fertilizer Increases Leaf Fresh Weight, Photosynthesis, and Fe-S Cluster Gene Expression in Peach (BioMed research international · 2020Article
- Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery.Scientific reports · 2019Article
- Cellular responses to reactive oxygen species are predicted from molecular mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
Iron-sulfur (Fe/S) cluster enzymes are crucial to life. Their assembly requires a suite of proteins, some of which are specific for particular subsets of Fe/S enzymes. One such protein is yeast Iba57p, which aconitase and certain radical S-adenosylmethionine enzymes require for activity. Iba57p homologs occur in all domains of life; they belong to the COG0354 protein family and are structurally similar to various folate-dependent enzymes. We therefore investigated the possible relationship between folates and Fe/S cluster enzymes using the Escherichia coli Iba57p homolog, YgfZ. NMR analysis confirmed that purified YgfZ showed stereoselective folate binding. Inactivating ygfZ reduced the activities of the Fe/S tRNA modification enzyme MiaB and certain other Fe/S enzymes, although not aconitase. When successive steps in folate biosynthesis were ablated, folE (lacking pterins and folates) and folP (lacking folates) mutants mimicked the ygfZ mutant in having low MiaB activities, whereas folE thyA mutants supplemented with 5-formyltetrahydrofolate (lacking pterins and depleted in dihydrofolate) and gcvP glyA mutants (lacking one-carbon tetrahydrofolates) had intermediate MiaB activities. These data indicate that YgfZ requires a folate, most probably tetrahydrofolate. Importantly, the ygfZ mutant was hypersensitive to oxidative stress and grew poorly on minimal media. COG0354 genes of bacterial, archaeal, fungal, protistan, animal, or plant origin complemented one or both of these growth phenotypes as well as the MiaB activity phenotype. Comparative genomic analysis indicated widespread functional associations between COG0354 proteins and Fe/S cluster metabolism. Thus COG0354 proteins have an ancient, conserved, folate-dependent function in the activity of certain Fe/S cluster enzymes.
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