ArticleProceedings of the National Academy of Sciences of the United States of America2010
Visualizing enveloping layer glycans during zebrafish early embryogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Investigation Into the Dynamics of the Cupula in the Vestibular Organ of Adult Zebrafish Using Metabolic Glycoengineering.Angewandte Chemie (International ed. in English) · 2026Article
- Click Chemistry-Based Hydrogels for Tissue Engineering.Gels (Basel, Switzerland) · 2025Review
- Exploring Metal-Free Click Reactions: New Frontiers in Glycochemistry and Bioconjugation.Bioconjugate chemistry · 2025Review
- Chemo-Selective Single-Cell Metabolomics Reveals the Spatiotemporal Behavior of Exogenous Pollutants During Xenopus Laevis Embryogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- In Situ Glycan Analysis and Editing in Living Systems.JACS Au · 2024Review
- α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation.Cell death & disease · 2023Article
- Cracking the "Sugar Code": A Snapshot ofFrontiers in plant science · 2021Review
- Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.Molecular omics · 2020Article
- Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy.Science advances · 2020Article
- The Emerging Role of the Mammalian Glycocalyx in Functional Membrane Organization and Immune System Regulation.Frontiers in cell and developmental biology · 2020Review
- Direct Visualization of Live Zebrafish Glycans via Single-Step Metabolic Labeling with Fluorophore-Tagged Nucleotide Sugars.Angewandte Chemie (International ed. in English) · 2019Review
- Microridges are apical epithelial projections formed of F-actin networks that organize the glycan layer.Scientific reports · 2019Article
- Optimization and Stability of Cell-Polymer Hybrids Obtained by "Clicking" Synthetic Polymers to Metabolically Labeled Cell Surface Glycans.Biomacromolecules · 2019Article
- Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications.Tissue engineering and regenerative medicine · 2018Review
- Achieving Controlled Biomolecule-Biomaterial Conjugation.Chemical reviews · 2018Review
- Imaging specific cellular glycan structures using glycosyltransferases via click chemistry.Glycobiology · 2018Article
- Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling.ACS chemical biology · 2017Review
- Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.Histochemistry and cell biology · 2017Review
- Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method.Chemical science · 2016Article
- Click labeling of unnatural sugars metabolically incorporated into viral envelope glycoproteins enables visualization of single particle fusion.Journal of virological methods · 2016Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Developmental events can be monitored at the cellular and molecular levels by using noninvasive imaging techniques. Among the biomolecules that might be targeted for imaging analysis, glycans occupy a privileged position by virtue of their primary location on the cell surface. We previously described a chemical method to image glycans during zebrafish larval development; however, we were unable to detect glycans during the first 24 hours of embryogenesis, a very dynamic period in development. Here we report an approach to the imaging of glycans that enables their visualization in the enveloping layer during the early stages of zebrafish embryogenesis. We microinjected embryos with azidosugars at the one-cell stage, allowed the zebrafish to develop, and detected the metabolically labeled glycans with copper-free click chemistry. Mucin-type O-glycans could be imaged as early as 7 hours postfertilization, during the gastrula stage of development. Additionally, we used a nonmetabolic approach to label sialylated glycans with an independent chemistry, enabling the simultaneous imaging of these two distinct classes of glycans. Imaging analysis of glycan trafficking revealed dramatic reorganization of glycans on the second time scale, including rapid migration to the cleavage furrow of mitotic cells. These studies yield insight into the biosynthesis and dynamics of glycans in the enveloping layer during embryogenesis and provide a platform for imaging other biomolecular targets by microinjection of appropriately functionalized biosynthetic precursors.
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