ArticleBiomaterials2010
Cultivating hepatocytes on printed arrays of HGF and BMP7 to characterize protective effects of these growth factors during in vitro alcohol injury.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Function of hepatocyte spheroids in bioactive microcapsules is enhanced by endogenous and exogenous hepatocyte growth factor.Bioactive materials · 2023Article
- Flow-Based Three-Dimensional Co-Culture Model for Long-Term Hepatotoxicity Prediction.Micromachines · 2019Article
- Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury.Cell research · 2019Article
- Tissue-specific transcriptional regulation of epithelial/endothelial and mesenchymal markers during renovascular hypertension.Molecular medicine reports · 2019Article
- Cross-Species Co-analysis of Prefrontal Cortex Chronic Ethanol Transcriptome Responses in Mice and Monkeys.Frontiers in molecular neuroscience · 2019Article
- Decreased BMP-7 and p-Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma.Oncology letters · 2018Article
- Engineered Livers for Infectious Diseases.Cellular and molecular gastroenterology and hepatology · 2018Review
- BMP Signalling at the Crossroad of Liver Fibrosis and Regeneration.International journal of molecular sciences · 2017Review
- Harnessing endogenous signals from hepatocytes using a low volume multi-well plate.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Ductular reaction-on-a-chip: Microfluidic co-cultures to study stem cell fate selection during liver injury.Scientific reports · 2016Article
- Microfluidic fabrication of bioactive microgels for rapid formation and enhanced differentiation of stem cell spheroids.Acta biomaterialia · 2016Article
- Using reconfigurable microfluidics to study the role of HGF in autocrine and paracrine signaling of hepatocytes.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Detecting transforming growth factor-β release from liver cells using an aptasensor integrated with microfluidics.Analytical chemistry · 2014Article
- Cell and tissue engineering for liver disease.Science translational medicine · 2014Review
- Local control of hepatic phenotype with growth factor-encoded surfaces.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Characterizing the effects of heparin gel stiffness on function of primary hepatocytes.Tissue engineering. Part A · 2013Article
- Micropatterning of ECM Proteins on Glass Substrates to Regulate Cell Attachment and Proliferation.Avicenna journal of medical biotechnology · 2013Article
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Hepatocyte growth factor overexpression ameliorates liver inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis.Hepatology international · 2012Article
- Cell signals influencing hepatic fibrosis.International journal of hepatology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The goal of the present study was to investigate hepato-protective effects of growth factor (GF) arrays during alcohol injury. Hepatocyte growth factor (HGF) and bone morphogenetic protein (BMP)7 were mixed with collagen (I) and robotically printed onto standard glass slides to create arrays of 500 microm diameter spots. Primary rat hepatocytes were seeded on top of the arrays forming clusters corresponding in size to the underlying protein spots. Cell arrays were then injured in culture by exposure to 100 mm ethanol for 48 h. Hepatocytes residing on GF spots were found to have less apoptosis then cells cultured on collagen-only spots. Least apoptosis (0.3% as estimated by TUNEL assay) was observed on HGF/BMP7/collagen spots whereas most apoptosis (17.3%) was seen on collagen-only arrays. Interestingly, the extent of alcohol-induced apoptosis in hepatocytes varied based on the concentration of printed GF. In addition to preventing apoptosis, printed GFs contributed to maintenance of epithelial phenotype during alcohol injury as evidenced by higher levels of E-cadherin expression in HGF-protected hepatocytes. Importantly, GF microarrays could be used to investigate heterotypic interactions in the context of liver injury. To highlight this, stellate cells - nonparenchymal liver cells involved in fibrosis - were added to hepatocytes residing on arrays of either HGF/collagen or collagen-only spots. Exposure of these cocultures to ethanol followed by RT-PCR analysis revealed that stellate cells residing alongside HGF-protected hepatocytes were significantly less activated (less fibrotic) compared to controls. Overall, our results demonstrate that GF microarray format can be used to screen anti-fibrotic and anti-apoptotic effects of growth factors as well as to investigate how signals delivered to a specific cell type modulate heterotypic cellular interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.