ArticleMolecular microbiology2010
Disruption of chemoreceptor signalling arrays by high levels of CheW, the receptor-kinase coupling protein.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- 1-Aminocyclopropane-1-Carboxylate and Its Chemoreceptor Drive Metabolic Reprogramming to Enhance Chemotactic Rhizocompetence inMicroorganisms · 2026Article
- Protein-protein interfaces in chemotaxis receptor complexes and their role in signaling.FEMS microbiology reviews · 2026Review
- Bacterial warfare at the molecular level:Current research in microbial sciences · 2026Article
- Hexameric rings of the scaffolding protein CheW enhance response sensitivity and cooperativity inScience signaling · 2022Article
- The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis.Frontiers in microbiology · 2020Article
- Noncritical Signaling Role of a Kinase-Receptor Interaction Surface in the Escherichia coli Chemosensory Core Complex.Journal of molecular biology · 2018Article
- The Transient Multidrug Resistance Phenotype ofFrontiers in microbiology · 2017Article
- SOS System Induction Inhibits the Assembly of Chemoreceptor Signaling Clusters in Salmonella enterica.PloS one · 2016Article
- Molecular Interaction and Cellular Location of RecA and CheW Proteins inFrontiers in microbiology · 2016Article
- Protein Connectivity in Chemotaxis Receptor Complexes.PLoS computational biology · 2015Article
- Bacterial chemoreceptors and chemoeffectors.Cellular and molecular life sciences : CMLS · 2015Review
- Internal sense of direction: sensing and signaling from cytoplasmic chemoreceptors.Microbiology and molecular biology reviews : MMBR · 2014Review
- Signalling-dependent interactions between the kinase-coupling protein CheW and chemoreceptors in living cells.Molecular microbiology · 2014Article
- RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica.PloS one · 2014Article
- Article
- Excitation and adaptation in bacteria-a model signal transduction system that controls taxis and spatial pattern formation.International journal of molecular sciences · 2013Article
- Conformational coupling between receptor and kinase binding sites through a conserved salt bridge in a signaling complex scaffold protein.PLoS computational biology · 2013Article
- A "trimer of dimers"-based model for the chemotactic signal transduction network in bacterial chemotaxis.Bulletin of mathematical biology · 2012Article
- Two CheW coupling proteins are essential in a chemosensory pathway of Borrelia burgdorferi.Molecular microbiology · 2012Article
- Molecular architecture of chemoreceptor arrays revealed by cryoelectron tomography of Escherichia coli minicells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
During chemotactic signalling by Escherichia coli, the small cytoplasmic CheW protein couples the histidine kinase CheA to chemoreceptor control. Although essential for assembly and operation of receptor signalling complexes, CheW in stoichiometric excess disrupts chemotactic behaviour. To explore the mechanism of the CheW excess effect, we measured the physiological consequences of high cellular levels of wild-type CheW and of several CheW variants with reduced or enhanced binding affinities for receptor molecules. We found that high levels of CheW interfered with trimer assembly, prevented CheA activation, blocked cluster formation, disrupted chemotactic ability and elevated receptor methylation levels. The severity of these effects paralleled the receptor-binding affinities of the CheW variants. Because trimer formation may be an obligate step in the assembly of ternary signalling complexes and higher-order receptor arrays, we suggest that all CheW excess effects stem from disruption of trimer assembly. We propose that the CheW-binding sites in receptor dimers overlap their trimer contact sites and that high levels of CheW saturate the receptor-binding sites, preventing trimer assembly. The CheW-trapped receptor dimers seem to be improved substrates for methyltransferase reactions, but cannot activate CheA or assemble into clusters, processes that are essential for chemotactic signalling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.