ArticlePloS one2010
Investigating the epigenetic effects of a prototype smoke-derived carcinogen in human cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Epigenetic alterations induced by genotoxic occupational and environmental human chemical carcinogens: A systematic literature review.Mutation research. Reviews in mutation researchPooled it
- Exploring the Utility of Long Non-Coding RNAs for Assessing the Health Consequences of Vaping.International journal of molecular sciences · 2024Review
- Influence of Benzo(a)pyrene on Different Epigenetic Processes.International journal of molecular sciences · 2021Review
- A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Environmental Epigenetics: Crossroad between Public Health, Lifestyle, and Cancer Prevention.BioMed research international · 2015Review
- A novel method for identification and quantification of consistently differentially methylated regions.PloS one · 2014Article
- Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development.Environmental toxicology and pharmacology · 2013Article
- Mammalian cells acquire epigenetic hallmarks of human cancer during immortalization.Nucleic acids research · 2013Article
- Epigenomic analysis of lung adenocarcinoma reveals novel DNA methylation patterns associated with smoking.OncoTargets and therapy · 2013Article
- Whole DNA methylome profiling in mice exposed to secondhand smoke.Epigenetics · 2012Article
- Genome-scale analysis of DNA methylation in lung adenocarcinoma and integration with mRNA expression.Genome research · 2012Article
- Epigenetics and lifestyle.Epigenomics · 2011Review
- Unveiling the methylation status of CpG dinucleotides in the substituted segment of the human p53 knock-in (Hupki) mouse genome.Molecular carcinogenesis · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Global loss of DNA methylation and locus/gene-specific gain of DNA methylation are two distinct hallmarks of carcinogenesis. Aberrant DNA methylation is implicated in smoking-related lung cancer. In this study, we have comprehensively investigated the modulation of DNA methylation consequent to chronic exposure to a prototype smoke-derived carcinogen, benzo[a]pyrene diol epoxide (B[a]PDE), in genomic regions of significance in lung cancer, in normal human cells. We have used a pulldown assay for enrichment of the CpG methylated fraction of cellular DNA combined with microarray platforms, followed by extensive validation through conventional bisulfite-based analysis. Here, we demonstrate strikingly similar patterns of DNA methylation in non-transformed B[a]PDE-treated cells vs control using high-throughput microarray-based DNA methylation profiling confirmed by conventional bisulfite-based DNA methylation analysis. The absence of aberrant DNA methylation in our model system within a timeframe that precedes cellular transformation suggests that following carcinogen exposure, other as yet unknown factors (secondary to carcinogen treatment) may help initiate global loss of DNA methylation and region-specific gain of DNA methylation, which can, in turn, contribute to lung cancer development. Unveiling the initiating events that cause aberrant DNA methylation in lung cancer has tremendous public health relevance, as it can help define future strategies for early detection and prevention of this highly lethal disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.