ArticlePLoS genetics2010
A comprehensive genome-wide map of autonomously replicating sequences in a naive genome.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- DNA bending mediated by ORC is essential for replication licensing in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Checkpoint kinase interaction with DNA polymerase alpha regulates replication progression during stress.Wellcome open research · 2023Article
- Stimulation of adaptive gene amplification by origin firing under replication fork constraint.Nucleic acids research · 2022Article
- Improved Production ofCurrent issues in molecular biology · 2021Article
- Various short autonomously replicating sequences from the yeastCurrent research in microbial sciences · 2021Article
- Computational prediction of species-specific yeast DNA replication origin via iterative feature representation.Briefings in bioinformatics · 2021Article
- Conservation of a DNA Replication Motif among Phylogenetically Distant Budding Yeast Species.Genome biology and evolution · 2021Article
- Humanizing the yeast origin recognition complex.Nature communications · 2021Article
- Autonomously Replicating Linear Plasmids That Facilitate the Analysis of Replication Origin Function inmSphere · 2019Article
- The evolution of the temporal program of genome replication.Nature communications · 2018Article
- DNA replication timing influences gene expression level.The Journal of cell biology · 2017Article
- Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.Microbial cell factories · 2016Article
- Chromosome Duplication in Saccharomyces cerevisiae.Genetics · 2016Review
- Genome-wide replication landscape of Candida glabrata.BMC biology · 2015Article
- Recent advances in the genome-wide study of DNA replication origins in yeast.Frontiers in microbiology · 2015Article
- Article
- GC-rich DNA elements enable replication origin activity in the methylotrophic yeast Pichia pastoris.PLoS genetics · 2014Article
- An autonomously replicating sequence for use in a wide range of budding yeasts.FEMS yeast research · 2014Article
- Replication-fork dynamics.Cold Spring Harbor perspectives in biology · 2014Review
- Replisome stall events have shaped the distribution of replication origins in the genomes of yeasts.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Eukaryotic chromosomes initiate DNA synthesis from multiple replication origins. The machinery that initiates DNA synthesis is highly conserved, but the sites where the replication initiation proteins bind have diverged significantly. Functional comparative genomics is an obvious approach to study the evolution of replication origins. However, to date, the Saccharomyces cerevisiae replication origin map is the only genome map available. Using an iterative approach that combines computational prediction and functional validation, we have generated a high-resolution genome-wide map of DNA replication origins in Kluyveromyces lactis. Unlike other yeasts or metazoans, K. lactis autonomously replicating sequences (KlARSs) contain a 50 bp consensus motif suggestive of a dimeric structure. This motif is necessary and largely sufficient for initiation and was used to dependably identify 145 of the up to 156 non-repetitive intergenic ARSs projected for the K. lactis genome. Though similar in genome sizes, K. lactis has half as many ARSs as its distant relative S. cerevisiae. Comparative genomic analysis shows that ARSs in K. lactis and S. cerevisiae preferentially localize to non-syntenic intergenic regions, linking ARSs with loci of accelerated evolutionary change.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.