ArticleProceedings of the National Academy of Sciences of the United States of America2010
Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 114 citations in OpenAlex.
- S267P mutation of OxyR regulator inSynthetic and systems biotechnology · 2026Article
- Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering.Microbial cell factories · 2024Review
- Systematic investigation of TetR-family transcriptional regulators and their roles on lignocellulosic inhibitor acetate tolerance inFrontiers in bioengineering and biotechnology · 2024Article
- Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress.Microbial cell factories · 2023Article
- Regulation of biofilm formation inFrontiers in bioengineering and biotechnology · 2023Article
- Impact of hfq and sigE on the tolerance of Zymomonas mobilis ZM4 to furfural and acetic acid stresses.PloS one · 2020Article
- The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance ofFrontiers in bioengineering and biotechnology · 2020Article
- Development and characterization of acidic-pH-tolerant mutants ofBiotechnology for biofuels · 2020Article
- Transcriptomic Profiles ofFrontiers in microbiology · 2020Article
- EngineeredBiotechnology for biofuels · 2019Article
- Prediction and characterization of promoters and ribosomal binding sites ofBiotechnology for biofuels · 2019Article
- Measures of single- versus multiple-round translation argue against a mechanism to ensure coupling of transcription and translation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Complete genome sequence and the expression pattern of plasmids of the model ethanologenBiotechnology for biofuels · 2018Article
- Sodium Acetate Responses inFrontiers in microbiology · 2018Review
- Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance inBiotechnology for biofuels · 2018Article
- Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms.Frontiers in bioengineering and biotechnology · 2018Article
- Effect of acetic acid on ethanol production by Zymomonas mobilis mutant strains through continuous adaptation.BMC biotechnology · 2017Article
- Draft Genome Sequencing of Ascomycetes YeastGenome announcements · 2017Article
- Identification and Characterization of 5' Untranslated Regions (5'UTRs) inFrontiers in microbiology · 2017Article
- Zymomonas mobilis as a model system for production of biofuels and biochemicals.Microbial biotechnology · 2016Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of systems biology tools holds promise for rational industrial microbial strain development. Here, we characterize a Zymomonas mobilis mutant (AcR) demonstrating sodium acetate tolerance that has potential importance in biofuel development. The genome changes associated with AcR are determined using microarray comparative genome sequencing (CGS) and 454-pyrosequencing. Sanger sequencing analysis is employed to validate genomic differences and to investigate CGS and 454-pyrosequencing limitations. Transcriptomics, genetic data and growth studies indicate that over-expression of the sodium-proton antiporter gene nhaA confers the elevated AcR sodium acetate tolerance phenotype. nhaA over-expression mostly confers enhanced sodium (Na(+)) tolerance and not acetate (Ac(-)) tolerance, unless both ions are present in sufficient quantities. NaAc is more inhibitory than potassium and ammonium acetate for Z. mobilis and the combination of elevated Na(+) and Ac(-) ions exerts a synergistic inhibitory effect for strain ZM4. A structural model for the NhaA sodium-proton antiporter is constructed to provide mechanistic insights. We demonstrate that Saccharomyces cerevisiae sodium-proton antiporter genes also contribute to sodium acetate, potassium acetate, and ammonium acetate tolerances. The present combination of classical and systems biology tools is a paradigm for accelerated industrial strain improvement and combines benefits of few a priori assumptions with detailed, rapid, mechanistic studies.
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