ArticleJournal of clinical microbiology2010
Comparison of the Eragen Multi-Code Respiratory Virus Panel with conventional viral testing and real-time multiplex PCR assays for detection of respiratory viruses.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Enterovirus-Rhinovirus-Induced Acute Respiratory Distress Syndrome in Adults: A Case Report and Short Literature Review.Case reports in infectious diseases · 2023Article
- Viral and Atypical Bacterial Detection in Young Nepalese Children Hospitalized with Severe Pneumonia.Microbiology spectrum · 2021Article
- SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy.Virology journal · 2021Article
- Research Progress of Coronavirus Based on Bibliometric Analysis.International journal of environmental research and public health · 2020Article
- Characteristics and Outcomes of Coronavirus Infection in Children: The Role of Viral Factors and an Immunocompromised State.Journal of the Pediatric Infectious Diseases Society · 2019Article
- Virological and Immunological Outcomes of Coinfections.Clinical microbiology reviews · 2018Review
- Human rhinovirus C infection is associated with asthma in children determined by xTAG respiratory viral panel FAST.Virologica Sinica · 2017Article
- Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis.American journal of respiratory and critical care medicine · 2016Article
- Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex xTAG® RVP fast assay for the detection of respiratory viruses.Journal of medical virology · 2016Article
- Review of Non-Bacterial Infections in Respiratory Medicine: Viral Pneumonia.Archivos de bronconeumologia · 2015Review
- Viral etiology of community-acquired pneumonia among adolescents and adults with mild or moderate severity and its relation to age and severity.BMC infectious diseases · 2015Article
- Ribonucleosides for an artificially expanded genetic information system.The Journal of organic chemistry · 2014Article
- Viral infection is not uncommon in adult patients with severe hospital-acquired pneumonia.PloS one · 2014Article
- Structural insights into DNA replication without hydrogen bonds.Journal of the American Chemical Society · 2013Article
- New molecular virus detection methods and their clinical value in lower respiratory tract infections in children.Paediatric respiratory reviews · 2013Review
- Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection.Diagnostic microbiology and infectious disease · 2013Article
- Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses.Journal of virological methods · 2012Article
- Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay.BMC infectious diseases · 2012Article
- Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- Evaluation of the PrimerDesign™ genesig real-time reverse transcription-polymerase chain reaction assay and the INFINITI® Respiratory Viral Panel Plus assay for the detection of human metapneumovirus in Kuwait.Diagnostic microbiology and infectious disease · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
High-throughput multiplex assays for respiratory viruses are an important step forward in diagnostic virology. We compared one such assay, the PLx Multi-Code Respiratory Virus Panel (PLx-RVP), manufactured by Eragen Biosciences, Inc. (Madison, WI), with conventional virologic testing, consisting of fluorescent-antibody staining plus testing with the R-mix system and fibroblast tube cultures. The test set consisted of 410 archived respiratory specimens, mostly nasopharyngeal swabs, including 210 that had been positive by conventional testing for a balanced selection of common respiratory viruses. Specimens yielding discrepant results were evaluated using a panel of respiratory virus PCR assays developed, characterized, and validated with clinical specimens. PLx-RVP increased the total rate of detection of viruses by 35.8%, and there was a 25.7% increase in the rate of detection of positive specimens. Reference PCR assay results corroborated the PLx-RVP result for 54 (82%) of 66 discrepancies with conventional testing. Of the 12 specimens with discrepancies between PLx-RVp and the reference PCRs, 6 were positive for rhinovirus by PLx-RVP and the presence of rhinovirus was confirmed by nucleotide sequencing. The remaining six specimens included five in which the PLx-RVP failed to detect parainfluenza virus and one in which the detection of influenza A virus by PLx-RVP could not be confirmed by the reference PCR. Taking the results of the reference PCR assay results into account, the sensitivities of the PLx-RVP for individual viruses ranged from 94 to 100% and the specificities ranged from 99 to 100%. We conclude that PLx-RVP is a highly accurate system for the detection of respiratory viruses and significantly improves the rate of detection of these viruses compared to that by conventional virologic testing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.