ArticleJournal of immunology (Baltimore, Md. : 1950)2010
Essential role for TLR9 in prime but not prime-boost plasmid DNA vaccination to activate dendritic cells and protect from lethal viral infection.
Article in Journal of immunology (Baltimore, Md. : 1950), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity.Human vaccines & immunotherapeutics · 2025Article
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- Tumor Immunity and Immunotherapy for HPV-Related Cancers.Cancer discovery · 2021Review
- Intranodal administration of mRNA encoding nucleoprotein provides cross-strain immunity against influenza in mice.Journal of translational medicine · 2019Article
- Advancements in DNA vaccine vectors, non-mechanical delivery methods, and molecular adjuvants to increase immunogenicity.Human vaccines & immunotherapeutics · 2017Review
- Molecular mechanisms for enhanced DNA vaccine immunogenicity.Expert review of vaccines · 2016Review
- Mucosal immunization with high-mobility group box 1 in chitosan enhances DNA vaccine-induced protection against coxsackievirus B3-induced myocarditis.Clinical and vaccine immunology : CVI · 2013Article
- DNA vaccines: a simple DNA sensing matter?Human vaccines & immunotherapeutics · 2013Review
- Review
- Cytokines as biomarkers of nanoparticle immunotoxicity.Chemical Society reviews · 2013Review
- The future of human DNA vaccines.Journal of biotechnology · 2012Review
- Nucleic acid sensing at the interface between innate and adaptive immunity in vaccination.Nature reviews. Immunology · 2012Review
- Single chain MHC I trimer-based DNA vaccines for protection against Listeria monocytogenes infection.Vaccine · 2012Article
- Technologies for enhanced efficacy of DNA vaccines.Expert review of vaccines · 2012Review
- Plasmid vector-linked maturation of natural killer (NK) cells is coupled to antigen-dependent NK cell activation during DNA-based immunization in mice.Journal of virology · 2011Article
- Dendritic cell maturation and chemotaxis is regulated by TRPM2-mediated lysosomal Ca2+ release.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Article
- Contrasting roles for TLR ligands in HIV-1 pathogenesis.PloS one · 2010Article
- Vaccines for COVID-19: perspectives from nucleic acid vaccines to BCG as delivery vector system.Microbes and infectionReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
One of the requirements for efficient vaccination against infection is to achieve the best combination of an adequate adjuvant with the antigenic information to deliver. Although plasmid DNA is a promising tool bearing the unique potential to activate humoral and cellular immunity, an actual challenge is to increase plasmid immunogenicity in human vaccination protocols in which efficacy has proven rather limited. Previous work showed that the bacterial DNA backbone of the plasmid has potent adjuvant properties because it contains CpG motifs that are particular activating nucleotidic sequences. Among TLRs, which are key sensors of microbial products, TLR9 can detect CpG motifs and confer activation of APCs, such as dendritic cells. However, whether the immunogenic properties of plasmid DNA involve TLR9 signaling has not been clearly established. In the current study, we demonstrate that TLR9 determines the effectiveness of vaccination against lethal lymphocytic choriomeningitis virus infection using plasmid DNA in a prime, but not prime-boost, vaccination regimen. Furthermore, we provide evidence that the presence of TLR9 in dendritic cells is necessary for effective and functional priming of virus-specific CD8+ T cells upon plasmid exposure in vitro or single-dose vaccination in vivo. Therefore, at single or low vaccine doses that are often used in human-vaccination protocols, CpG/TLR9 interactions participate in the immunogenicity of plasmid DNA. These results suggest that the TLR9 signaling pathway is involved in the efficacy of plasmid vaccination; therefore, it should remain a focus in the development or amelioration of vaccines to treat infections in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.