ArticleJournal of immunology (Baltimore, Md. : 1950)2010
Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo.
Article in Journal of immunology (Baltimore, Md. : 1950), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- The nonamer code for RAG-mediated recombination in vivo.Nucleic acids research · 2025Article
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Regulated large-scale nucleosome density patterns and precise nucleosome positioning correlate with V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination.Cell reports · 2016Article
- Influence of a CTCF-Dependent Insulator on Multiple Aspects of Enhancer-Mediated Chromatin Organization.Molecular and cellular biology · 2015Article
- The proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination.PloS one · 2015Article
- Noncore RAG1 regions promote Vβ rearrangements and αβ T cell development by overcoming inherent inefficiency of Vβ recombination signal sequences.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Transcription-coupled eviction of histones H2A/H2B governs V(D)J recombination.The EMBO journal · 2013Article
- Control of Ig gene assembly: lessons from premature activation.The EMBO journal · 2013Article
- DNA double-strand breaks relieve USF-mediated repression of Dβ2 germline transcription in developing thymocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Long-distance regulation of fetal V(δ) gene segment TRDV4 by the Tcrd enhancer.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Orchestrating T-cell receptor α gene assembly through changes in chromatin structure and organization.Immunologic research · 2011Article
- Recombination centres and the orchestration of V(D)J recombination.Nature reviews. Immunology · 2011Review
- Control of V(D)J Recombination through Transcriptional Elongation and Changes in Locus Chromatin Structure and Nuclear Organization.Genetics research international · 2011Article
- Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination.The Journal of experimental medicine · 2010Article
- Regulation of antigen receptor gene assembly by genetic-epigenetic crosstalk.Seminars in immunology · 2010Review
- The center of accessibility: Dβ control of V(D)J recombination.Archivum immunologiae et therapiae experimentalis · 2010Review
- Epigenetics and the adaptive immune response.Molecular aspects of medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Accessibility of chromosomal recombination signal sequences to the RAG protein complex is known to be essential for V(D)J recombination at Ag receptor loci in vivo. Previous studies have addressed the roles of cis-acting regulatory elements and germline transcription in the covalent modification of nucleosomes at Ag receptor loci. However, a detailed picture of nucleosome organization at accessible and inaccessible recombination signal sequences has been lacking. In this study, we have analyzed the nucleosome organization of accessible and inaccessible Tcrb and Tcra alleles in primary murine thymocytes in vivo. We identified highly positioned arrays of nucleosomes at Dbeta, Jbeta, and Jalpha segments and obtained evidence indicating that positioning is established at least in part by the regional DNA sequence. However, we found no consistent positioning of nucleosomes with respect to recombination signal sequences, which could be nucleosomal or internucleosomal even in their inaccessible configurations. Enhancer- and promoter-dependent accessibility was characterized by diminished abundance of certain nucleosomes and repositioning of others. Moreover, some changes in nucleosome positioning and abundance at Jalpha61 were shown to be a direct consequence of germline transcription. We suggest that enhancer- and promoter-dependent transcription generates optimal recombinase substrates in which some nucleosomes are missing and others are covalently modified.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.