ArticleBiophysical journal2010
DNA translocation and unzipping through a nanopore: some geometrical effects.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Epx4 Nanopore With Multiple Constrictions for Single-Molecule Identification.Small methods · 2026Article
- Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA.Analytical chemistry · 2026Article
- Article
- Ionic transport through a protein nanopore: a Coarse-Grained Molecular Dynamics Study.Scientific reports · 2019Article
- Detecting topological variations of DNA at single-molecule level.Nature communications · 2019Article
- Comparative biosensing of glycosaminoglycan hyaluronic acid oligo- and polysaccharides using aerolysin andThe European physical journal. E, Soft matter · 2018Article
- Kinetics of T3-DNA Ligase-Catalyzed Phosphodiester Bond Formation Measured Using the α-Hemolysin Nanopore.ACS nano · 2016Article
- MOSAIC: A Modular Single-Molecule Analysis Interface for Decoding Multistate Nanopore Data.Analytical chemistry · 2016Article
- Base Flipping within the α-Hemolysin Latch Allows Single-Molecule Identification of Mismatches in DNA.Journal of the American Chemical Society · 2016Article
- Click Addition of a DNA Thread to the N-Termini of Peptides for Their Translocation through Solid-State Nanopores.ACS nano · 2015Article
- Probing molecular pathways for DNA orientational trapping, unzipping and translocation in nanopores by using a tunable overhang sensor.Nanoscale · 2014Article
- Temperature and electrolyte optimization of the α-hemolysin latch sensing zone for detection of base modification in double-stranded DNA.Biophysical journal · 2014Article
- Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.ACS applied materials & interfaces · 2014Article
- Interactions of the human telomere sequence with the nanocavity of the α-hemolysin ion channel reveal structure-dependent electrical signatures for hybrid folds.Journal of the American Chemical Society · 2013Article
- Electrical Current Signatures of DNA Base Modifications in Single Molecules Immobilized in the α-Hemolysin Ion Channel.Israel journal of chemistry · 2013Article
- On the Lubensky-Nelson model of polymer translocation through nanopores.Biophysical journal · 2012Article
- Crown ether-electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Unzipping kinetics of duplex DNA containing oxidized lesions in an α-hemolysin nanopore.Journal of the American Chemical Society · 2012Article
- Quantitative analysis of the nanopore translocation dynamics of simple structured polynucleotides.Biophysical journal · 2012Article
- Sequence-specific single-molecule analysis of 8-oxo-7,8-dihydroguanine lesions in DNA based on unzipping kinetics of complementary probes in ion channel recordings.Journal of the American Chemical Society · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This article explores the role of some geometrical factors on the electrophoretically driven translocations of macromolecules through nanopores. In the case of asymmetric pores, we show how the entry requirements and the direction of translocation can modify the information content of the blocked ionic current as well as the transduction of the electrophoretic drive into a mechanical force. To address these effects we studied the translocation of single-stranded DNA through an asymmetric alpha-hemolysin pore. Depending on the direction of the translocation, we measure the capacity of the pore to discriminate between both DNA orientations. By unzipping DNA hairpins from both sides of the pores we show that the presence of single strand or double strand in the pore can be discriminated based on ionic current levels. We also show that the transduction of the electrophoretic drive into a denaturing mechanical force depends on the local geometry of the pore entrance. Eventually we discuss the application of this work to the measurement of energy barriers for DNA unzipping as well as for protein binding and unfolding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.