ArticleBMC developmental biology2010
Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters.
Article in BMC developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A nuclear targeting approach enables efficient transgenesis in the milkweed bugbioRxiv : the preprint server for biology · 2026Article
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
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- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
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- Context-dependent coordination of movement in Tribolium castaneum larvae.The Journal of experimental biology · 2025Article
- Generating and testing the efficacy of reagents for CRISPR/Cas9 homology directed repair-based manipulations in Tribolium.Journal of insect science (Online) · 2024Article
- Temporal control of RNAi reveals both robust and labile feedback loops in the segmentation clock of the red flour beetle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species.Scientific reports · 2024Article
- An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae.Cell reports methods · 2024Article
- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- NHA1 is a cation/proton antiporter essential for the water-conserving functions of the rectal complex inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Global Gene Expression Analysis Reveals Complex Cuticle Organization of the Tribolium Compound Eye.Genome biology and evolution · 2023Article
- Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum.Insect molecular biology · 2022Article
- Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology.BMC genomics · 2022Article
- Review
- Screens in fly and beetle reveal vastly divergent gene sets required for developmental processes.BMC biology · 2022Article
- EfficientFrontiers in genome editing · 2022Article
- Mutually Exclusive Expression of Closely Related Odorant-Binding Proteins 9A and 9B in the Antenna of the Red Flour BeetleBiomolecules · 2021Article
- A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.Scientific reports · 2021Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe red flour beetle Tribolium castaneum has developed into an insect model system second only to Drosophila. Moreover, as a coleopteran it represents the most species-rich metazoan taxon which also includes many pest species. The genetic toolbox for Tribolium research has expanded in the past years but spatio-temporally controlled misexpression of genes has not been possible so far.
resultsHere we report the establishment of the GAL4/UAS binary expression system in Tribolium castaneum. Both GAL4 Delta and GAL4VP16 driven by the endogenous heat shock inducible promoter of the Tribolium hsp68 gene are efficient in activating reporter gene expression under the control of the Upstream Activating Sequence (UAS). UAS driven ubiquitous tGFP fluorescence was observed in embryos within four hours after activation while in-situ hybridization against tGFP revealed expression already after two hours. The response is quick in relation to the duration of embryonic development in Tribolium - 72 hours with segmentation being completed after 24 hours - which makes the study of early embryonic processes possible using this system. By comparing the efficiency of constructs based on Tribolium, Drosophila, and artificial core promoters, respectively, we find that the use of endogenous core promoters is essential for high-level expression of transgenic constructs.
conclusionsWith the established GAL4/UAS binary expression system, ectopic misexpression approaches are now feasible in Tribolium. Our results support the contention that high-level transgene expression usually requires endogenous regulatory sequences, including endogenous core promoters in Tribolium and probably also other model systems.
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