ArticleBMC plant biology2010
Identification of a new 130 bp cis-acting element in the TsVP1 promoter involved in the salt stress response from Thellungiella halophila.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 71 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Bioinformatics and Functional Analysis of OsASMT1 Gene in Response to Abiotic Stress.Biochemical genetics · 2025Article
- Genome-Wide Identification, Phylogenetic Evolution, and Abiotic Stress Response Analyses of the Late Embryogenesis Abundant Gene Family in the Alpine Cold-Tolerant MedicinalInternational journal of molecular sciences · 2025Article
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- Genome-Wide Identification and Salt Stress Response Analysis of the bZIP Transcription Factor Family in Sugar Beet.International journal of molecular sciences · 2022Article
- Impact of Nanomaterials on the Regulation of Gene Expression and Metabolomics of Plants under Salt Stress.Plants (Basel, Switzerland) · 2022Review
- Identification of a 193 bp promoter region of TaNRX1-D gene from common wheat that contributes to osmotic or ABA stress inducibility in transgenic Arabidopsis.Genes & genomics · 2021Article
- Responses of two dominant desert plant species to the changes in groundwater depth in hinterland natural oasis, Tarim Basin.Ecology and evolution · 2021Article
- Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar.BMC plant biology · 2021Article
- Mechanisms of Plant Responses and Adaptation to Soil Salinity.Innovation (Cambridge (Mass.)) · 2020Review
- Sugar Beet (International journal of molecular sciences · 2020Article
- Review
- Characterization of a novel LmSAP gene promoter from Lobularia maritima: Tissue specificity and environmental stress responsiveness.PloS one · 2020Article
- Analysis of Arabidopsis thaliana HKT1 and Eutrema salsugineum/botschantzevii HKT1;2 Promoters in Response to Salt Stress in Athkt1:1 Mutant.Molecular biotechnology · 2019Article
- A specific allele of MYB14 in grapevine correlates with high stilbene inducibility triggered by AlPlant cell reports · 2019Article
- Identification and characterization ofPeerJ · 2019Article
- Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.PloS one · 2019Article
- Halophytism: What Have We Learnt FromPlant physiology · 2018Review
- Stress Inducible Overexpression ofFrontiers in chemistry · 2018Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalt stress is one of the major abiotic stresses affecting plant growth and productivity. Vacuolar H+-pyrophosphatase (H+-PPase) genes play an important role in salt stress tolerance in multiple species.
resultsIn this study, the promoter from the vacuolar H+-pyrophosphatase from Thellungiella halophila (TsVP1) was cloned and compared with the AVP1 promoter from Arabidopsis thaliana. Sequence analysis indicated that these two promoters had seven similar motifs at similar positions. To determine which tissues the two promoters are active in, transgenic plants were produced with expression of the GUS reporter gene under the control of one of the promoters. In transgenic Arabidopsis with the TsVP1 promoter, the GUS reporter gene had strong activity in almost all tissues except the seeds and the activity was induced in both shoots and roots, especially in the root tips, when treated with salt stress. Such induction was not found in transgenic Arabidopsis with the AVP1 promoter. By analyzing different 5' deletion mutants of the TsVP1 promoter, an 856 bp region (-2200 to -1344) was found to contain enhancer elements that increased gene expression levels. Two AAATGA motifs, which may be the key elements for the anther specific expression profile, in the deleted TsVP1 promoters (PT2 to PT6) were also identified. A 130 bp region (-667 to -538) was finally identified as the key sequence for the salt stress response by analyzing the different mutants both with and without salt stress. GUS transient assay in tobacco leaves suggested the 130 bp region was sufficient for the salt stress response. Bioinformatic analysis also revealed that there may be novel motifs in this region that are the key elements for the salt stress responsive activity of the TsVP1 promoter.
conclusionsThe TsVP1 promoter had strong activity in almost all tissues except the seeds. In addition, its activity was induced by salt stress in leaves and roots, especially in root tips. A 130 bp region (-667 to -538) was identified as the key region for responding to salt stress.
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