ArticleJournal of perinatal medicine2010
Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods.
Article in Journal of perinatal medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women.Frontiers in cellular and infection microbiology · 2013Pooled it
- Prostaglandin and prostamide concentrations in amniotic fluid of women with spontaneous labor at term with and without clinical chorioamnionitis.Prostaglandins, leukotrienes, and essential fatty acids · 2020Trial
- Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis.Molecular microbiology · 2025Article
- Human papillomavirus 16 mitigatesmSphere · 2025Article
- Unveiling the placental bacterial microbiota: implications for maternal and infant health.Frontiers in physiology · 2025Review
- Molecular evidence that GBS early neonatal sepsis results from ascending infection: comparative hybrid genomics analyses show that microorganisms in the vaginal ecosystem, amniotic fluid, chorioamniotic membranes, and neonatal blood are the same.Journal of perinatal medicine · 2024Article
- Review
- Early life gut microbiome in children following spontaneous preterm birth and maternal preeclampsia.iScience · 2023Article
- Thymic stromal lymphopoietin participates in the host response to intra-amniotic inflammation leading to preterm labor and birth.Human immunology · 2023Article
- Article
- Identification of the pore-forming and binding domains of the Sneathia vaginalis cytopathogenic toxin A.PloS one · 2023Article
- Questioning the fetal microbiome illustrates pitfalls of low-biomass microbial studies.Nature · 2023Review
- Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation.Frontiers in immunology · 2022Article
- Preterm labor is characterized by a high abundance of amniotic fluid prostaglandins in patients with intra-amniotic infection or sterile intra-amniotic inflammation.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2021Article
- Bacteria in the amniotic fluid without inflammation: early colonization vs. contamination.Journal of perinatal medicine · 2021Article
- Review
- The Vaginal Virome-Balancing Female Genital Tract Bacteriome, Mucosal Immunity, and Sexual and Reproductive Health Outcomes?Viruses · 2020Review
- Identification of a Cytopathogenic Toxin from Sneathia amnii.Journal of bacteriology · 2020Article
- Review
- Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
objectiveInfection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia. METHOD OF STUDY: Amniotic fluid (AF) from 62 subjects with preeclampsia, not in labor, was analyzed with both cultivation and molecular methods. Broad-range and group-specific PCR assays targeting small subunit ribosomal DNA, or other gene sequences, from bacteria, fungi and archaea were used. Results were correlated with measurements of host inflammatory response, including AF white blood cell count and AF concentrations of glucose, interleukin-6 (IL-6) and MMP-8.
results1) The rate of MIAC in preeclampsia was 1.6% (1/62) based on cultivation techniques, 8% (5/62) based on PCR, and 9.6% (6/62) based on the combined results of both methods; 2) among the six patients diagnosed with MIAC, three had a positive PCR for Sneathia/Leptotrichia spp.; and 3) patients with MIAC were more likely to have evidence of an inflammatory response in the amniotic cavity than those without MIAC, as determined by a higher median AF IL-6 [1.65 ng/mL interquartile range (IQR): 0.35-4.62 vs. 0.22 ng/mL IQR: 0.12-0.51; P=0.002).
conclusionThe prevalence of MIAC in preeclampsia is low, suggesting that intra-amniotic infection plays only a limited role in preeclampsia. However, the unexpectedly high number of positive AF specimens for Sneathia/Leptotrichia warrants further investigation.
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