ArticlePloS one2010
Organophosphorus pesticides decrease M2 muscarinic receptor function in guinea pig airway nerves via indirect mechanisms.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Organophosphorus Pesticides as Modulating Substances of Inflammation through the Cholinergic Pathway.International journal of molecular sciences · 2022Review
- Differences in neurotoxic outcomes of organophosphorus pesticides revealedFrontiers in toxicology · 2022Article
- Metformin prevents airway hyperreactivity in rats with dietary obesity.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Pioglitazone prevents obesity-related airway hyperreactivity and neuronal MAmerican journal of physiology. Lung cellular and molecular physiology · 2021Article
- Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay.International journal of environmental research and public health · 2020Article
- Organophosphorus Pesticides Induce Cytokine Release from Differentiated Human THP1 Cells.American journal of respiratory cell and molecular biology · 2019Article
- Mechanisms of organophosphorus pesticide toxicity in the context of airway hyperreactivity and asthma.American journal of physiology. Lung cellular and molecular physiology · 2018Review
- Neurotoxicity in acute and repeated organophosphate exposure.Toxicology · 2018Review
- Eosinophil and airway nerve interactions in asthma.Journal of leukocyte biology · 2018Review
- Impact of chronic exposure to the pesticide chlorpyrifos on respiratory parameters and sleep apnea in juvenile and adult rats.PloS one · 2018Article
- Ozone-induced eosinophil recruitment to airways is altered by antigen sensitization and tumor necrosis factor-Physiological reports · 2017Article
- Effects of phosalone consumption via feeding with or without sodium bentonite on performance, blood metabolites and its transition to milk of Iranian Baluchi sheep.Journal of animal science and technology · 2017Article
- Phosalone-induced inflammation and oxidative stress in the colon: Evaluation and treatment.World journal of gastroenterology · 2016Article
- Electrochemical detection of malathion pesticide using acetylcholinesterase biosensor based on glassy carbon electrode modified with conducting polymer film.Environmental science and pollution research international · 2016Article
- Urinary Dialkyl Phosphate Concentrations and Lung Function Parameters in Adolescents and Adults: Results from the Canadian Health Measures Survey.Environmental health perspectives · 2016Article
- Environmental exposure to pesticides and respiratory health.European respiratory review : an official journal of the European Respiratory Society · 2015Review
- Chlorpyrifos exposure and respiratory health among adolescent agricultural workers.International journal of environmental research and public health · 2014Article
- Oxidative stress resulting from exposure of a human salivary gland cells to paraoxon: an in vitro model for organophosphate oral exposure.Toxicology in vitro : an international journal published in association with BIBRA · 2014Article
- Macrophage TNF-α mediates parathion-induced airway hyperreactivity in guinea pigs.American journal of physiology. Lung cellular and molecular physiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundEpidemiological studies link organophosphorus pesticide (OP) exposures to asthma, and we have shown that the OPs chlorpyrifos, diazinon and parathion cause airway hyperreactivity in guinea pigs 24 hr after a single subcutaneous injection. OP-induced airway hyperreactivity involves M2 muscarinic receptor dysfunction on airway nerves independent of acetylcholinesterase (AChE) inhibition, but how OPs inhibit neuronal M2 receptors in airways is not known. In the central nervous system, OPs interact directly with neurons to alter muscarinic receptor function or expression; therefore, in this study we tested whether the OP parathion or its oxon metabolite, paraoxon, might decrease M2 receptor function on peripheral neurons via similar direct mechanisms. METHODOLOGY/PRINCIPAL
findingsIntravenous administration of paraoxon, but not parathion, caused acute frequency-dependent potentiation of vagally-induced bronchoconstriction and increased electrical field stimulation (EFS)-induced contractions in isolated trachea independent of AChE inhibition. However, paraoxon had no effect on vagally-induced bradycardia in intact guinea pigs or EFS-induced contractions in isolated ileum, suggesting mechanisms other than pharmacologic antagonism of M2 receptors. Paraoxon did not alter M2 receptor expression in cultured cells at the mRNA or protein level as determined by quantitative RT-PCR and radio-ligand binding assays, respectively. Additionally, a biotin-labeled fluorophosphonate, which was used as a probe to identify molecular targets phosphorylated by OPs, did not phosphorylate proteins in guinea pig cardiac membranes that were recognized by M2 receptor antibodies. CONCLUSIONS/SIGNIFICANCE: These data indicate that neither direct pharmacologic antagonism nor downregulated expression of M2 receptors contributes to OP inhibition of M2 function in airway nerves, adding to the growing evidence of non-cholinergic mechanisms of OP neurotoxicity.
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Registered trials
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