ArticlePloS one2010
A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Engineering kidney developmental trajectory using culture boundary conditions.Nature communications · 2025Article
- Preeclampsia impedes foetal kidney development by delivering placenta-derived exosomes to glomerular endothelial cells.Cell communication and signaling : CCS · 2023Article
- Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches.bioRxiv : the preprint server for biology · 2023Article
- The developing murine kidney actively negotiates geometric packing conflicts to avoid defects.Developmental cell · 2023Article
- Effect of Hypoxia on Branching Characteristics and Cell Subpopulations during Kidney Organ Culture.Bioengineering (Basel, Switzerland) · 2022Article
- Calreticulin Deficiency Disturbs Ribosome Biogenesis and Results in Retardation in Embryonic Kidney Development.International journal of molecular sciences · 2021Article
- The biomechanical basis of biased epithelial tube elongation in lung and kidney development.Development (Cambridge, England) · 2021Article
- The Last Half Century of Fish Explant and Organ Culture.Zebrafish · 2021Article
- Article
- In developing mouse kidneys, orientation of loop of Henle growth is adaptive and guided by long-range cues from medullary collecting ducts.Journal of anatomy · 2019Article
- Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Sebinger Culture: A System Optimized for Morphological Maturation and Imaging of Cultured Mouse Metanephric Primordia.Bio-protocol · 2018Article
- Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy.PloS one · 2018Article
- BMP7 dose-dependently stimulates proliferation and cadherin-11 expression via ERK and p38 in a murine metanephric mesenchymal cell line.Physiological reports · 2017Article
- Dissection and Culture of Mouse Embryonic Kidney.Journal of visualized experiments : JoVE · 2017Article
- Novel fixedDevelopment (Cambridge, England) · 2017Article
- A strategy for generating kidney organoids: Recapitulating the development in human pluripotent stem cells.Developmental biology · 2016Review
- Detection of initial angiogenesis from dorsal aorta into metanephroi and elucidation of its role in kidney development.Regenerative therapy · 2016Article
- Transport of organic anions and cations in murine embryonic kidney development and in serially-reaggregated engineered kidneys.Scientific reports · 2015Article
- Organ In Vitro Culture: What Have We Learned about Early Kidney Development?Stem cells international · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.