ArticleThe Journal of cell biology2010
Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 254 papers.
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Who cites it
254 citing papers in PubMed.
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- Conserved function of a RasGEF-mediated pathway in the metabolic compensation of the circadian clock.The FEBS journal · 2025Article
- Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis.Plant physiology · 2025Article
- Cleavage of MEP-1 by DPF-3 Reveals Novel Substrate Specificity and Its Impact on Reproductive Fitness.bioRxiv : the preprint server for biology · 2025Article
- Steric repulsion counteracts ER-to-lipid droplet protein movement.Science advances · 2025Article
- Locking CBL TKBD in its native conformation presents a novel therapeutic opportunity in mutant CBL-dependent leukemia.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Structural characterization and inhibition of the interaction between ch-TOG and TACC3.The Journal of cell biology · 2025Article
- INCREASED DNA METHYLATION 3 forms a potential chromatin remodelling complex with HAIRPLUS to regulate DNA methylation and trichome development in tomato.The Plant journal : for cell and molecular biology · 2025Article
- Comprehensive Evaluation of Advanced Imputation Methods for Proteomic Data Acquired via the Label-Free Approach.International journal of molecular sciences · 2024Article
- K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors.Life science alliance · 2024Article
- Cell-cell transfer of adaptation traits benefits kin and actor in a cooperative microbe.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF.Nature genetics · 2024Article
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- Multiparameter screen optimizes immunoprecipitation.BioTechniques · 2024Article
- STIM1 translocation to the nucleus protects cells from DNA damage.Nucleic acids research · 2024Article
- Phototropin2 3'UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase.BMC plant biology · 2024Article
194 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein interactions are involved in all cellular processes. Their efficient and reliable characterization is therefore essential for understanding biological mechanisms. In this study, we show that combining bacterial artificial chromosome (BAC) TransgeneOmics with quantitative interaction proteomics, which we call quantitative BAC-green fluorescent protein interactomics (QUBIC), allows specific and highly sensitive detection of interactions using rapid, generic, and quantitative procedures with minimal material. We applied this approach to identify known and novel components of well-studied complexes such as the anaphase-promoting complex. Furthermore, we demonstrate second generation interaction proteomics by incorporating directed mutational transgene modification and drug perturbation into QUBIC. These methods identified domain/isoform-specific interactors of pericentrin- and phosphorylation-specific interactors of TACC3, which are necessary for its recruitment to mitotic spindles. The scalability, simplicity, cost effectiveness, and sensitivity of this method provide a basis for its general use in small-scale experiments and in mapping the human protein interactome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.