ArticleProceedings of the National Academy of Sciences of the United States of America2010
Biological imaging with 4D ultrafast electron microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Imaging the Transverse Component of Optical Near-Fields in Resonant Photonic Structures.ACS photonics · 2026Article
- Ultrafast 4D Scanning Transmission Electron Microscopy for Imaging of Localized Optical Fields.ACS photonics · 2025Article
- Relativistic ultrafast electron diffraction at high repetition rates.Structural dynamics (Melville, N.Y.) · 2023Article
- Imaging the field inside nanophotonic accelerators.Nature communications · 2023Article
- Sensing ultrashort electronic coherent beating at conical intersections by single-electron pulses.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Photon Statistics of Incoherent Cathodoluminescence with Continuous and Pulsed Electron Beams.ACS photonics · 2021Article
- Near field excited state imaging via stimulated electron energy gain spectroscopy of localized surface plasmon resonances in plasmonic nanorod antennas.Scientific reports · 2020Article
- 4D electron microscopy of T cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- From structure to structural dynamics: Ahmed Zewail's legacy.Structural dynamics (Melville, N.Y.) · 2017Article
- Femtosecond electron imaging of defect-modulated phonon dynamics.Nature communications · 2016Article
- Infrared PINEM developed by diffraction in 4D UEM.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Photon gating in four-dimensional ultrafast electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Quantum coherent optical phase modulation in an ultrafast transmission electron microscope.Nature · 2015Article
- Dynamics deep from the core.Structural dynamics (Melville, N.Y.) · 2015Article
- Graphene-layered steps and their fields visualized by 4D electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Imaging interactions of metal oxide nanoparticles with macrophage cells by ultra-high resolution scanning electron microscopy techniques.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Electron microscopy of specimens in liquid.Nature nanotechnology · 2011Review
- Fully hydrated yeast cells imaged with electron microscopy.Biophysical journal · 2011Article
- Faster, sharper electron microscopy.Nature methods · 2010Article
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3 authors.
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Abstract
Advances in the imaging of biological structures with transmission electron microscopy continue to reveal information at the nanometer length scale and below. The images obtained are static, i.e., time-averaged over seconds, and the weak contrast is usually enhanced through sophisticated specimen preparation techniques and/or improvements in electron optics and methodologies. Here we report the application of the technique of photon-induced near-field electron microscopy (PINEM) to imaging of biological specimens with femtosecond (fs) temporal resolution. In PINEM, the biological structure is exposed to single-electron packets and simultaneously irradiated with fs laser pulses that are coincident with the electron pulses in space and time. By electron energy-filtering those electrons that gained photon energies, the contrast is enhanced only at the surface of the structures involved. This method is demonstrated here in imaging of protein vesicles and whole cells of Escherichia coli, both are not absorbing the photon energy, and both are of low-Z contrast. It is also shown that the spatial location of contrast enhancement can be controlled via laser polarization, time resolution, and tomographic tilting. The high-magnification PINEM imaging provides the nanometer scale and the fs temporal resolution. The potential of applications is discussed and includes the study of antibodies and immunolabeling within the cell.
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