ArticleGenes & development2010
Proper regulation of a sperm-specific cis-nat-siRNA is essential for double fertilization in Arabidopsis.
Article in Genes & development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 179 citations in OpenAlex.
- LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen.Plant, cell & environment · 2026Article
- Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.Plant cell reports · 2026Review
- In Vivo Maternal Haploid Induction by Disrupting KOKOPELLI in Medicago truncatula.Plant biotechnology journal · 2026Article
- The mysterious dialogue between the embryo, endosperm, and seed coat, and its implications for seed traits.Journal of experimental botany · 2026Review
- Genome-wide identification, characterization, and expression analysis of the DMP gene family in Chinese cabbage (Brassica rapa ssp. pekinensis).BMC plant biology · 2025Article
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- Genome-Wide Identification and Characterization ofInternational journal of molecular sciences · 2025Article
- Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development.Annals of botany · 2024Article
- An elastic proteinaceous envelope encapsulates the early Arabidopsis embryo.Development (Cambridge, England) · 2023Article
- In planta haploid induction by kokopelli mutants.Plant physiology · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization.eLife · 2023Article
- Genome-wide identification, characterization and expression analysis of AGO, DCL, and RDR families in Chenopodium quinoa.Scientific reports · 2023Article
- Genetic basis of maize maternal haploid induction beyondFrontiers in plant science · 2023Article
- The Multiverse of Plant Small RNAs: How Can We Explore It?International journal of molecular sciences · 2022Review
- Biogenesis, Functions, Interactions, and Resources of Non-Coding RNAs in Plants.International journal of molecular sciences · 2022Review
- Non-coding RNAs fine-tune the balance between plant growth and abiotic stress tolerance.Frontiers in plant science · 2022Review
- Recent Advances in Plant Gene Silencing Methods.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in Arabidopsis.Developmental cell · 2021Article
- Plant Long Noncoding RNAs: New Players in the Field of Post-Transcriptional Regulations.Non-coding RNA · 2021Review
33 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural cis-antisense siRNAs (cis-nat-siRNAs) are a recently characterized class of small regulatory RNAs that are widespread in eukaryotes. Despite their abundance, the importance of their regulatory activity is largely unknown. The only functional role for eukaryotic cis-nat-siRNAs that has been described to date is in environmental stress responses in plants. Here we demonstrate that cis-nat-siRNA-based regulation plays key roles in Arabidopsis reproductive function, as it facilitates gametophyte formation and double fertilization, a developmental process of enormous agricultural value. We show that male gametophytic kokopelli (kpl) mutants display frequent single-fertilization events, and that KPL and a inversely transcribed gene, ARIADNE14 (ARI14), which encodes a putative ubiquitin E3 ligase, generate a sperm-specific nat-siRNA pair. In the absence of KPL, ARI14 RNA levels in sperm are increased and fertilization is impaired. Furthermore, ARI14 transcripts accumulate in several siRNA biogenesis pathway mutants, and overexpression of ARI14 in sperm phenocopies the reduced seed set of the kokopelli mutants. These results extend the regulatory capacity of cis-nat-siRNAs to development by identifying a role for cis-nat-siRNAs in controlling sperm function during double fertilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.