ArticleJournal of experimental botany2010
Characterization of a trimeric light-harvesting complex in the diatom Phaeodactylum tricornutum built of FcpA and FcpE proteins.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Towards Microalgal Biorefinery: Multiproduct Fractionation ofMarine drugs · 2026Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- DiatOmicBase: a versatile gene-centered platform for mining functional omics data in diatom research.The Plant journal : for cell and molecular biology · 2025Article
- Structural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states.Plant communications · 2024Article
- Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics.Scientific reports · 2022Article
- Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum.MicrobiologyOpen · 2021Article
- Light Harvesting in Fluctuating Environments: Evolution and Function of Antenna Proteins across Photosynthetic Lineage.Plants (Basel, Switzerland) · 2021Article
- Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum.Scientific reports · 2019Article
- Dynamic Changes between Two LHCX-Related Energy Quenching Sites Control Diatom Photoacclimation.Plant physiology · 2018Article
- How reduced excitonic coupling enhances light harvesting in the main photosynthetic antennae of diatoms.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Red-light phenotype in a marine diatom involves a specialized oligomeric red-shifted antenna and altered cell morphology.Scientific reports · 2017Article
- Utilization of light by fucoxanthin-chlorophyll-binding protein in a marine centric diatom, Chaetoceros gracilis.Photosynthesis research · 2015Article
- Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage.The Plant journal : for cell and molecular biology · 2015Article
- Supramolecular organization of fucoxanthin-chlorophyll proteins in centric and pennate diatoms.Photosynthesis research · 2014Article
- Molecular dynamics of the diatom thylakoid membrane under different light conditions.Photosynthesis research · 2012Review
- Modulation of the multilamellar membrane organization and of the chiral macrodomains in the diatom Phaeodactylum tricornutum revealed by small-angle neutron scattering and circular dichroism spectroscopy.Photosynthesis research · 2012Article
- Structure of PSI, PSII and antennae complexes from yellow-green alga Xanthonema debile.Photosynthesis research · 2011Article
- Evidence for the existence of one antenna-associated, lipid-dissolved and two protein-bound pools of diadinoxanthin cycle pigments in diatoms.Plant physiology · 2010Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fucoxanthin chlorophyll proteins (Fcps), the light-harvesting antennas of heterokont algae, are encoded by a multigene family and are highly similar with respect to their molecular masses as well as to their pigmentation, making it difficult to purify single Fcps. In this study, a hexa-histidine tag was genetically added to the C-terminus of the FcpA protein of the pennate diatom Phaeodactylum tricornutum. A transgenic strain expressing the recombinant His-tagged FcpA protein in addition to the endogenous wild type Fcps was created. This strategy allowed, for the first time, the purification of a specific, stable trimeric Fcp complex. In addition, a pool of various trimeric Fcps was also purified from the wild-type cells using sucrose density gradient ultracentrifugation and gel filtration. In both the His-tagged and the wild-type Fcps, excitation energy coupling between fucoxanthin and chlorophyll a was intact and the existence of a chlorophyll a/fucoxanthin excitonic dimer was demonstrated using circular dichroism spectroscopy. Mass spectrometric analyses of the trimeric His-tagged complex indicated that it is composed of FcpA and FcpE polypeptides. It is confirmed here that a trimer is the basic organizational unit of Fcps in P. tricornutum. From circular dichroism spectra, it is proposed that the organization of the pigments on the polypeptide backbone of Fcps is a conserved feature in the case of chlorophyll a/c containing algae.
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