ArticleBMC plant biology2010
The role of actin isoforms in somatic embryogenesis in Norway spruce.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Please inhibit responsibly: Natural and synthetic actin toxins as useful tools in cell biology.Molecular biology of the cell · 2025Review
- Establishment of embryogenic Pinus thunbergii Parl. suspension cultures: growth parameters, dynamic analysis, and plant regenerative capacities.BMC plant biology · 2024Article
- Proteomic and Metabolic Analysis ofInternational journal of molecular sciences · 2023Article
- Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.Scientific reports · 2021Article
- Physiological Basis of Smut Infectivity in the Early Stages of Sugar Cane Colonization.Journal of fungi (Basel, Switzerland) · 2021Review
- Profiles of Endogenous Phytohormones Over the Course of Norway Spruce Somatic Embryogenesis.Frontiers in plant science · 2018Article
- Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.Frontiers in plant science · 2018Review
- Overexpression of PaNAC03, a stress induced NAC gene family transcription factor in Norway spruce leads to reduced flavonol biosynthesis and aberrant embryo development.BMC plant biology · 2017Article
- Medicago truncatula and Glycine max: Different Drought Tolerance and Similar Local Response of the Root Nodule Proteome.Journal of proteome research · 2015Article
- Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.Plant signaling & behavior · 2015Article
- Article
- Deciphering small noncoding RNAs during the transition from dormant embryo to germinated embryo in Larches (Larix leptolepis).PloS one · 2013Article
- Unveiling interactions among mitochondria, caspase-like proteases, and the actin cytoskeleton during plant programmed cell death (PCD).PloS one · 2013Article
- Multiple actin isotypes in plants: diverse genes for diverse roles?Frontiers in plant science · 2012Article
- Organisation and regulation of the cytoskeleton in plant programmed cell death.Cell death and differentiation · 2011Review
- The F-actin cytoskeleton in syncytia from non-clonal progenitor cells.Protoplasma · 2011Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSomatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs.
resultsApplication of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was greatly enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B.
conclusionsWe show that manipulating actin in specific cell types in somatic embryos using Lat B treatment accelerated and even synchronized the development of somatic embryos and may be of practical use in biotechnology.
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