ArticleMicrobial cell factories2010
Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- A Review of Chondroitin Sulfate's Preparation, Properties, Functions, and Applications.Molecules (Basel, Switzerland) · 2023Review
- Simultaneous production ofHeliyon · 2023Article
- Heterologous production of chondroitin.Biotechnology reports (Amsterdam, Netherlands) · 2022Review
- Misincorporation of Galactose by Chondroitin Synthase ofBiomolecules · 2020Article
- N-glycolyl chondroitin synthesis using metabolically engineered E. coli.AMB Express · 2020Article
- Molecular Dissection of dH3w, A Fluorescent Peptidyl Sensor for Zinc and Mercury.Sensors (Basel, Switzerland) · 2020Article
- Denatured lysozyme-coated carbon nanotubes: a versatile biohybrid material.Scientific reports · 2019Article
- Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest.AMB Express · 2017Article
- An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli.Microbial cell factories · 2017Article
- New insight into chondroitin and heparosan-like capsular polysaccharide synthesis by profiling of the nucleotide sugar precursors.Bioscience reports · 2017Article
- Heparin and related polysaccharides: synthesis using recombinant enzymes and metabolic engineering.Applied microbiology and biotechnology · 2015Review
- Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.FEMS microbiology reviews · 2014Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide.
resultsA random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encoding chondroitin polymerase (K4CP), with a change from arginine to glutamine. A docking analysis to explain the increased productivity of the K4CP enzyme is presented.
conclusionThe enhanced chondroitin production by the E. coli K4 mutant reported here shows the validity of the strain improvement strategy for more cost-friendly fermentative processes in the production of this pharmaceutically important but so-far expensive polysaccharide.
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