ArticleThe Journal of parasitology2010
Long double-stranded RNA produces specific gene downregulation in Giardia lamblia.
Article in The Journal of parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis.Genetics · 2024Review
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Exosome Biogenesis in the Protozoa ParasiteCells · 2019Article
- Resistance formation to nitro drugs in Giardia lamblia: No common markers identified by comparative proteomics.International journal for parasitology. Drugs and drug resistance · 2019Article
- Robust and stable transcriptional repression in Giardia using CRISPRi.Molecular biology of the cell · 2019Article
- Article
- Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia.Nature communications · 2016Article
- Article
- A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia.RNA (New York, N.Y.) · 2011Article
- Gene regulation in Giardia lambia involves a putative microRNA derived from a small nucleolar RNA.PLoS neglected tropical diseases · 2011Article
- RNA interference in protozoan parasites: achievements and challenges.Eukaryotic cell · 2011Review
- The emerging world of small silencing RNAs in protozoan parasites.Trends in parasitology · 2011Review
- Characterizing ncRNAs in Human Pathogenic Protists Using High-Throughput Sequencing Technology.Frontiers in genetics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
In many eukaryotes, the introduction of double-stranded RNA (dsRNA) into cells triggers the degradation of mRNAs through a post-transcriptional gene-silencing mechanism called RNA interference or RNAi. In the present study, we found that endogenous long-dsRNA was substantially more effective at producing interference than endogenous, or exogenous, short-dsRNA expression in Giardia lamblia . The effects of this interference were not evident in the highly expressed protein tubulin or the stage-specific cyst wall protein 2. However, long-dsRNA caused potent and specific interference in the medium subunits of adaptins, the RNA-dependent RNA polymerase, and the exogenous green fluorescence protein. Our results suggest that the ability of dsRNA antisense to inhibit the expression of these specific types of proteins is indicative of a gene-specific mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.