ArticleAmino acids2010
Mild reductions in cytosolic NADP-dependent isocitrate dehydrogenase activity result in lower amino acid contents and pigmentation without impacting growth.
Article in Amino acids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Malate matters: disrupting bacterial-type phosphoenolpyruvate carboxylase (BTPC) rewires tomato fruit development.Plant physiology · 2026Article
- Coordinated regulation of amino acid metabolism and transport in cereal nitrogen remobilization for grain filling.Frontiers in plant science · 2026Review
- PbrNAC34a- PbrMYB3/65- PbrACO2 cascade plays a role in citrate difference between the pericarp and cortex tissues of pear (P. bretschneideri Rehd.) fruit.Molecular horticulture · 2025Article
- Light-Independent Nitrogen Assimilation in Plant Leaves: Nitrate Incorporation into Glutamine, Glutamate, Aspartate, and Asparagine Traced byPlants (Basel, Switzerland) · 2020Review
- A Multi-Species Analysis Defines Anaplerotic Enzymes and Amides as Metabolic Markers for Ammonium Nutrition.Frontiers in plant science · 2020Article
- Isotopic labelling reveals the efficient adaptation of wheat root TCA cycle flux modes to match carbon demand under ammonium nutrition.Scientific reports · 2019Article
- Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?Frontiers in plant science · 2018Review
- Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement.Protoplasma · 2016Article
- Article
- Evolution and functional implications of the tricarboxylic acid cycle as revealed by phylogenetic analysis.Genome biology and evolution · 2014Article
- Plant metabolic modeling: achieving new insight into metabolism and metabolic engineering.The Plant cell · 2014Review
- Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.Plant physiology · 2014Article
- Global transcriptome analysis of AtPAP2--overexpressing Arabidopsis thaliana with elevated ATP.BMC genomics · 2013Article
- Transcriptomic analysis of the role of carboxylic acids in metabolite signaling in Arabidopsis leaves.Plant physiology · 2013Article
- Phosphonate analogs of 2-oxoglutarate perturb metabolism and gene expression in illuminated Arabidopsis leaves.Frontiers in plant science · 2012Article
- Functional genomics tools applied to plant metabolism: a survey on plant respiration, its connections and the annotation of complex gene functions.Frontiers in plant science · 2012Article
- Recommendations for reporting metabolite data.The Plant cell · 2011Article
- Antisense inhibition of the iron-sulphur subunit of succinate dehydrogenase enhances photosynthesis and growth in tomato via an organic acid-mediated effect on stomatal aperture.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach. The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves. However, biochemical analyses revealed that the transgenic lines displayed a considerable shift in metabolism, being characterized by decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H. The plants showed little change in photosynthesis with the exception of a minor decrease in maximum photosynthetic efficiency (F (v)/F (m)), and a small decrease in growth compared to the wild type. These results reveal that even small changes in cytosolic NADP-dependent isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acids. These data are discussed within the context of current models for the role of the various isoforms of isocitrate dehydrogenase within plant amino acid metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.