ArticlePlant molecular biology2010
Compositional and transcriptional analyses of reduced zein kernels derived from the opaque2 mutation and RNAi suppression.
Article in Plant molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Regulatory factors of starch metabolism in cereal endosperm.Plant molecular biology · 2026Review
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- Antisense RNA (asRNA) technology: the concept and applications in crop improvement and sustainable agriculture.Molecular biology reports · 2023Review
- Identification of a candidate gene underlying qHKW3, a QTL for hundred-kernel weight in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.Scientific reports · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Cytosolic malate dehydrogenase 4 modulates cellular energetics and storage reserve accumulation in maize endosperm.Plant biotechnology journal · 2020Article
- Temporal profiling of essential amino acids in developing maize kernel of normal,Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Intra-Kernel Reallocation of Proteins in Maize Depends on VP1-Mediated Scutellum Development and Nutrient Assimilation.The Plant cell · 2019Article
- The Zea mays mutants opaque2 and opaque16 disclose lysine change in waxy maize as revealed by RNA-Seq.Scientific reports · 2019Article
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- Maize opaque mutants are no longer so opaque.Plant reproduction · 2018Review
- Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum.Plant physiology · 2018Article
- Article
- Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.Journal of experimental botany · 2016Article
- Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.The Plant cell · 2015Article
- Protein accumulation in aleurone cells, sub-aleurone cells and the center starch endosperm of cereals.Plant cell reports · 2014Review
- Seed-specific expression of a lysine-rich protein gene, GhLRP, from cotton significantly increases the lysine content in maize seeds.International journal of molecular sciences · 2014Article
- Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich δ-zein in transgenic soybeans.Frontiers in plant science · 2014Article
- Proteome balancing of the maize seed for higher nutritional value.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corn protein is largely made up of a group of nutritionally limited storage proteins known as zein. The reduction of zein can be achieved by a transcriptional mutation, opaque2 (o2), or a transgene targeting zein through RNA interference (RNAi). Zein reduction results in an increase of more nutritionally balanced non-zein proteins, and therefore enhance the overall quality of corn protein. In this study, the composition of mature kernels and the transcriptional profile of developing kernels of these two types of zein reduced kernels were compared. Both zein reduced kernels contained higher levels of lysine and tryptophan and free amino acids were 10-20-folds more abundant than the wild-type counterpart. We also found that free lysine contributed partially to the increased lysine in o2 kernels while protein-bound lysine was mainly responsible for the increased lysine in transgenic zein reduction (TZR) kernels. Although they had relatively similar gene expression patterns in developing endosperm, o2 kernels had greater transcriptional changes than TZR kernels in general. A number of transcripts that were specifically down-regulated in o2 were identified. Many promoter sequences of these transcripts contain putative O2 binding motifs, suggesting that their expression is directly regulated by O2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.