ArticleIntegrative biology : quantitative biosciences from nano to macro2010
Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.
Article in Integrative biology : quantitative biosciences from nano to macro, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
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- Complications of pulsed-field ablation for atrial fibrillation: a narrative structured review.Frontiers in cardiovascular medicine · 2026Review
- Overcoming the Challenges of Transmural Durable Lesions With PFA-Impact of Electrode Design and Contact Assessment.Pacing and clinical electrophysiology : PACE · 2026Review
- Prediction of major intravascular hemolysis during pulsed electric field ablation of atrial fibrillation using a pentaspline catheter.Journal of cardiovascular electrophysiology · 2024Article
- Impact of Pulsed Electric Field Ablation on His Bundle Conduction: A Preclinical Canine Study.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- Preclinical evaluation of reversible pulsed electrical field: electrophysiological and histological assessment of myocardium.Frontiers in cardiovascular medicine · 2024Article
- Pulsed-field ablation: Computational modeling of electric fields for lesion depth analysis.Heart rhythm O2 · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- CRISPR medicine for blood disorders: Progress and challenges in delivery.Frontiers in genome editing · 2022Review
- Parametric optimization of electric field strength for cancer electrochemotherapy on a chip-based model.Theranostics · 2018Article
- Magnetic tweezers-based 3D microchannel electroporation for high-throughput gene transfection in living cells.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Spatially variant red blood cell crenation in alternating current non-uniform fields.Biomicrofluidics · 2014Article
- Shape-dependent optoelectronic cell lysis.Angewandte Chemie (International ed. in English) · 2014Article
- Microfluidic electroporation for cellular analysis and delivery.Lab on a chip · 2013Review
- Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.Biomedical microdevices · 2013Article
- A theoretical study of single-cell electroporation in a microchannel.The Journal of membrane biology · 2013Article
- Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?Biomicrofluidics · 2013Article
- Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network.Lab on a chip · 2011Article
- Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy.Journal of lipid research · 2010Review
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Circulating tumor cells (CTCs) refer to cells that detach from a primary tumor, circulate in the blood stream, and may settle down at a secondary site and form metastases. The detection and characterization of CTCs are clinically useful for diagnosis and prognosis purposes. However, there has been very little work on purging CTCs from the blood. In this study, we systematically studied electroporation of tumor and blood cells in the context of selective purging and analysis of CTCs, using M109 and mouse blood cells as models. Electroporation is a simple and effective method for disruption of the cell membrane by applying an external electric field. We applied a microfluidic flow-through electroporation to process cells with various electroporation durations and field intensities. With duration of 100-300 ms, we found that the thresholds for electroporation-induced lysis started at 300-400 V cm(-1) for M109, 400-500 V cm(-1) for white blood cells and 1100-1200 V cm(-1) for red blood cells. Due to the substantial difference, we demonstrated the selective electroporation of tumor cells among blood cells and the scale-up of the flow-through electroporation devices for processing samples of millilitre volumes. Using Coherent Anti-stokes Raman Scattering (CARS) and fluorescence microscopy tools, we observed the dramatic increase in the size of the nucleus of a tumor cell in response to the applied field. We suggest that the nucleus expansion is a newly discovered mechanism responsible for rapid tumor cell death resulted from electroporation.
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