ArticleProceedings of the National Academy of Sciences of the United States of America2010
Near-isotropic 3D optical nanoscopy with photon-limited chromophores.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- ImprovedJournal of biomedical optics · 2023Article
- Reproducible Antigen Recognition by the Type I-F CRISPR-Cas System.The CRISPR journal · 2020Article
- ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range.Nature communications · 2018Article
- Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies.Scientific reports · 2018Article
- Spatial and spectral imaging of point-spread functions using a spatial light modulator.Optics communications · 2017Article
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Optical and force nanoscopy in microbiology.Nature microbiology · 2016Review
- High-resolution real-time dual-view imaging with multiple point of view microscopy.Biomedical optics express · 2016Article
- Optical tracking of nanoscale particles in microscale environments.Applied physics reviews · 2016Article
- Single-molecule spectroscopy and imaging over the decades.Faraday discussions · 2015Review
- Review
- Precisely and accurately localizing single emitters in fluorescence microscopy.Nature methods · 2014Review
- Fluorophore localization algorithms for super-resolution microscopy.Nature methods · 2014Review
- Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.Nature photonics · 2014Article
- Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE).Optics express · 2012Article
- Advances in light-based imaging of three-dimensional cellular ultrastructure.Current opinion in cell biology · 2012Review
- Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Optical nanoscopy: from acquisition to analysis.Annual review of biomedical engineering · 2012Review
- Motion analysis of live objects by super-resolution fluorescence microscopy.Computational and mathematical methods in medicine · 2012Review
- Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging approaches based on single molecule localization break the diffraction barrier of conventional fluorescence microscopy, allowing for bioimaging with nanometer resolution. It remains a challenge, however, to precisely localize photon-limited single molecules in 3D. We have developed a new localization-based imaging technique achieving almost isotropic subdiffraction resolution in 3D. A tilted mirror is used to generate a side view in addition to the front view of activated single emitters, allowing their 3D localization to be precisely determined for superresolution imaging. Because both front and side views are in focus, this method is able to efficiently collect emitted photons. The technique is simple to implement on a commercial fluorescence microscope, and especially suitable for biological samples with photon-limited chromophores such as endogenously expressed photoactivatable fluorescent proteins. Moreover, this method is relatively resistant to optical aberration, as it requires only centroid determination for localization analysis. Here we demonstrate the application of this method to 3D imaging of bacterial protein distribution and neuron dendritic morphology with subdiffraction resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.