ArticleJournal of bacteriology2010
Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 110 citations in OpenAlex.
- Adaptation without Dominance in Pseudomonas syringae Pathovars.Microbial ecology · 2025Article
- Genomic characterization ofAdvances in laboratory medicine · 2025Article
- Article
- Biofilm and Outer Membrane Vesicle Formation in ESKAPE Gram-Negative Bacteria: A Comprehensive Review.International journal of molecular sciences · 2025Review
- Identification and Validation of Promising Targets and Inhibitors of Biofilm Formation inPathogens (Basel, Switzerland) · 2025Article
- Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis.Communications biology · 2025Article
- RetS-mediated environmental sensing coordinates TetR-dependent regulation of type III secretion system and virulence inApplied and environmental microbiology · 2025Article
- CRISPR-driven enhanced hydrocarbon emulsification in an environmental Pseudomonas aeruginosa strain.Microbial cell factories · 2025Article
- VimR is a conserved master transcription regulator controlling virulence and metabolism in Pseudomonas.Nucleic acids research · 2025Article
- A Homolog of the Histidine Kinase RetS Controls the Synthesis of Alginates, PHB, Alkylresorcinols, and Motility in Azotobacter vinelandii.Current microbiology · 2024Article
- T6SS nuclease effectors inJournal of bacteriology · 2024Article
- Transcriptome profiling of type VI secretion system core geneMicrobiology spectrum · 2024Article
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
- Transcriptional organization and regulation of theMicrobiology (Reading, England) · 2023Article
- Genome-wide screen in human plasma identifies multifaceted complement evasion of Pseudomonas aeruginosa.PLoS pathogens · 2023Article
- tRNA modification enzyme MiaB connects environmental cues to activation of Pseudomonas aeruginosa type III secretion system.PLoS pathogens · 2022Article
- Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics.Signal transduction and targeted therapy · 2022Review
- Condensins are essential for Pseudomonas aeruginosa corneal virulence through their control of lifestyle and virulence programs.Molecular microbiology · 2022Article
- The Type VI Secretion Systems in Plant-Beneficial Bacteria Modulate Prokaryotic and Eukaryotic Interactions in the Rhizosphere.Frontiers in microbiology · 2022Article
- The type VI secretion system of Xanthomonas phaseoli pv. manihotis is involved in virulence and in vitro motility.BMC microbiology · 2021Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas syringae pv. syringae B728a is a resident on leaves of common bean, where it utilizes several well-studied virulence factors, including secreted effectors and toxins, to develop a pathogenic interaction with its host. The B728a genome was recently sequenced, revealing the presence of 1,297 genes with unknown function. This study demonstrates that a 29.9-kb cluster of genes in the B728a genome shares homology to the novel type VI secretion system (T6SS) locus recently described for other gram-negative bacteria. Western blot analyses showed that B728a secretes Hcp, a T6SS protein, in culture and that this secretion is dependent on clpV, a gene that likely encodes an AAA(+) ATPase. In addition, we have identified two B728a sensor kinases that have homology to the P. aeruginosa proteins RetS and LadS. We demonstrate that B728a RetS and LadS reciprocally regulate the T6SS and collectively modulate several virulence-related activities. Quantitative PCR analyses indicated that RetS and LadS regulate genes associated with the type III secretion system and that LadS controls the expression of genes involved in the production of the exopolysaccharides alginate and levan. These analyses also revealed that LadS and the hybrid sensor kinase GacS positively regulate the expression of a putative novel exopolysaccharide called Psl. Plate assays demonstrated that RetS negatively controls mucoidy, while LadS negatively regulates swarming motility. A mutation in retS affected B728a population levels on the surfaces of bean leaves. A model for the LadS and RetS control of B728a virulence activities is proposed.
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