ArticleJournal of bacteriology2010
Factors affecting variability in time between addition of nutrient germinants and rapid dipicolinic acid release during germination of spores of Bacillus species.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 103 citations in OpenAlex.
- A mathematical kinetic model of memory iniScience · 2026Article
- Sporulation at reduced water activity impairs germination kinetics ofApplied and environmental microbiology · 2025Article
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- Inactivation effect of extracts of gardenia fruit, licorice andFood science and biotechnology · 2025Article
- Influence of Sporulation Temperature on Germination and Growth ofFoods (Basel, Switzerland) · 2024Article
- Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches.Archives of microbiology · 2024Article
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
- Heat Activation and Inactivation of Bacterial Spores: Is There an Overlap?Applied and environmental microbiology · 2022Article
- Moderate High-Pressure Superdormancy inApplied and environmental microbiology · 2022Article
- Review
- Germination, Outgrowth, and Vegetative-Growth Kinetics of Dry-Heat-Treated Individual Spores of Bacillus Species.Applied and environmental microbiology · 2018Article
- Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff.Nature communications · 2018Article
- Single-cell analysis reveals individual spore responses to simulated space vacuum.NPJ microgravity · 2018Article
- Superdormant Spores as a Hurdle for Gentle Germination-Inactivation Based Spore Control Strategies.Frontiers in microbiology · 2018Article
- Assessing the Impact of Germination and Sporulation Conditions on the Adhesion of Bacillus Spores to Glass and Stainless Steel by Fluid Dynamic Gauging.Journal of food science · 2017Article
- Role of stereospecific nature of germinants in3 Biotech · 2017Article
- Analysis of the Germination of IndividualFrontiers in microbiology · 2017Article
- Article
- Characterization of the Dynamic Germination of Individual Clostridium difficile Spores Using Raman Spectroscopy and Differential Interference Contrast Microscopy.Journal of bacteriology · 2015Article
- The effects of heat activation on Bacillus spore germination, with nutrients or under high pressure, with or without various germination proteins.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The simultaneous nutrient germination of hundreds of individual wild-type spores of three Bacillus species and a number of Bacillus subtilis strains has been measured by two new methods, and rates of release of the great majority of the large pool of dipicolinic acid (DPA) from individual spores of B. subtilis strains has been measured by Raman spectroscopy with laser tweezers. The results from these analyses and published data have allowed a number of significant conclusions about the germination of spores of Bacillus species as follows. (i) The time needed for release of the great majority of a Bacillus spore's DPA once rapid DPA release had begun (DeltaT(release)) during nutrient germination was independent of the concentration of nutrient germinant used, the level of the germinant receptors (GRs) that recognize nutrient germinants used and heat activation prior to germination. Values for DeltaT(release) were generally 0.5 to 3 min at 25 to 37 degrees C for individual wild-type spores. (ii) Despite the conclusion above, germination of individual spores in populations was very heterogeneous, with some spores in wild-type populations completing germination > or = 15-fold slower than others. (iii) The major factor in the heterogeneity in germination of individual spores in populations was the highly variable lag time, T(lag), between mixing spores with nutrient germinants and the beginning of DeltaT(release). (iv) A number of factors decrease spores' T(lag) values including heat activation, increased levels of GRs/spore, and higher levels of nutrient germinants. These latter factors appear to affect the level of activated GRs/spore during nutrient germination. (v) The conclusions above lead to the simple prediction that a major factor causing heterogeneity in Bacillus spore germination is the number of functional GRs in individual spores, a number that presumably varies significantly between spores in populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.