ArticleJournal of bacteriology2010
The response regulator SprE (RssB) is required for maintaining poly(A) polymerase I-degradosome association during stationary phase.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Roles of the membrane-binding motif and the C-terminal domain of RNase E in localization and diffusion ineLife · 2025Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria.Life science alliance · 2023Article
- Regulation of mRNA decay inCritical reviews in biochemistry and molecular biology · 2022Article
- Transgenesis of mammalian PABP reveals mRNA polyadenylation as a general stress response mechanism in bacteria.iScience · 2021Article
- RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in Helicobacter pylori.Nucleic acids research · 2021Article
- Phase-separated bacterial ribonucleoprotein bodies organize mRNA decay.Wiley interdisciplinary reviews. RNA · 2020Review
- Bacterial RNA Degradosomes: Molecular Machines under Tight Control.Trends in biochemical sciences · 2020Review
- Extragenic Suppression of Elongation Factor P Gene Mutant Phenotypes in Erwinia amylovora.Journal of bacteriology · 2019Article
- RNA polyadenylation and its consequences in prokaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- RNase E and the High-Fidelity Orchestration of RNA Metabolism.Microbiology spectrum · 2018Review
- Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes.Nucleic acids research · 2018Article
- Sirtuin Lipoamidase Activity Is Conserved in Bacteria as a Regulator of Metabolic Enzyme Complexes.mBio · 2017Article
- Elucidation of pathways of ribosomal RNA degradation: an essential role for RNase E.RNA (New York, N.Y.) · 2016Article
- Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome.eLife · 2016Article
- Temporal Regulation of themSystems · 2016Article
- Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy.Methods in molecular biology (Clifton, N.J.) · 2016Article
- ComGA-RelA interaction and persistence in the Bacillus subtilis K-state.Molecular microbiology · 2015Article
- RNase E in the γ-Proteobacteria: conservation of intrinsically disordered noncatalytic region and molecular evolution of microdomains.Molecular genetics and genomics : MGG · 2015Article
- Initiation of mRNA decay in bacteria.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Poly(A) polymerase I (PAP I) is the enzyme responsible for the addition of poly(A) tails onto RNA molecules in Escherichia coli. Polyadenylation is believed to facilitate the destruction of such RNAs by the mRNA degradosome. Recently, it was discovered that the stationary-phase regulatory protein SprE (RssB) has a second function in the control of polyadenylation that is distinct from its known function in the regulated proteolysis of RpoS. In the work presented herein, we used a targeted proteomic approach to further investigate SprE's involvement in the polyadenylation pathway. Specifically, we used cryogenic cell lysis, immunopurifications on magnetic beads, and mass spectrometry to identify interacting partners of PAP I-green fluorescent protein. We provide the first in vivo evidence that PAP I interacts with the mRNA degradosome during both exponential and stationary phases and find that the degradosome can contain up to 10 different proteins under certain conditions. Moreover, we demonstrate that the majority of these PAP I interactions are formed via protein-protein interactions and that SprE plays an important role in the maintenance of the PAP I-degradosome association during stationary phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.