ArticleApplied and environmental microbiology2010
{gamma}-Glutamylmethylamide is an essential intermediate in the metabolism of methylamine by Methylocella silvestris.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.Applied and environmental microbiology · 2026Article
- Conserved pathway for homarine catabolism in environmental bacteria.Nature microbiology · 2026Article
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- Characterization of the TrimethylamineFrontiers in microbiology · 2022Article
- Aerobic bacterial methane synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Conserved Metabolic and Evolutionary Themes in Microbial Degradation of Carbamate Pesticides.Frontiers in microbiology · 2021Review
- Pathway engineering ofMetabolic engineering communications · 2020Article
- Facultative methanotrophs - diversity, genetics, molecular ecology and biotechnological potential: a mini-review.Microbiology (Reading, England) · 2020Review
- FermentativeMicroorganisms · 2020Article
- Evidence of Interdomain Ammonium Cross-Feeding From Methylamine- and Glycine Betaine-DegradingFrontiers in microbiology · 2020Article
- Carbaryl as a Carbon and Nitrogen Source: an Inducible Methylamine Metabolic Pathway at the Biochemical and Molecular Levels inApplied and environmental microbiology · 2018Article
- One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst.Scientific reports · 2018Article
- Draft Genome Sequences of Obligate Methylotrophs Methylovorus sp. Strain MM2 and Methylobacillus sp. Strain MM3, Isolated from Grassland Soil.Microbiology resource announcements · 2018Article
- Aerobic proteobacterial methylotrophs in Movile Cave: genomic and metagenomic analyses.Microbiome · 2018Article
- Fermentative Production ofFrontiers in bioengineering and biotechnology · 2018Article
- Interkingdom Cross-Feeding of Ammonium from Marine Methylamine-Degrading Bacteria to the Diatom Phaeodactylum tricornutum.Applied and environmental microbiology · 2016Article
- Draft Genome Sequences of Facultative Methylotrophs, Gemmobacter sp. Strain LW1 and Mesorhizobium sp. Strain 1M-11, Isolated from Movile Cave, Romania.Genome announcements · 2015Article
- C₁-Pathways in Methyloversatilis universalis FAM5: Genome Wide Gene Expression and Mutagenesis Studies.Microorganisms · 2015Article
- Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria.Microorganisms · 2015Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylocella silvestris BL2, a facultative methane utilizer, can grow on monomethylamine (MMA) as a sole carbon and nitrogen source. No activity of MMA dehydrogenase was detectable. Instead, this bacterium utilizes a methylated amino acid pathway (gamma-glutamylmethylamide [GMA] and N-methylglutamate [NMG]) for MMA metabolism. The activities of the two key enzymes in this pathway, GMA synthetase and NMG dehydrogenase, were found when the bacterium was grown on MMA. GMA was detected by high-performance liquid chromatography-mass spectrometry only when the bacterium was grown on MMA but not when it was grown on methanol. Proteomic analysis of soluble and membrane fractions of the proteome from MMA- and methanol-grown cultures revealed that an eight-gene cluster (Msil2632 to Msil2639) was induced by MMA and cotranscribed as an operon, as shown by reverse transcription-PCR. GMA-dissimilating enzyme activity was also detected when it was grown on MMA. Formaldehyde and ammonium production from GMA was dependent on glutamate but not on alpha-ketoglutarate. Marker exchange mutagenesis of a putative GMAS gene homologue (gmas, Msil2635) within this eight-gene cluster, with a kanamycin gene cassette, abolished growth of M. silvestris on MMA as either a sole carbon or a sole nitrogen source. Overall, our results suggest that gmas is essential in MMA metabolism by M. silvestris.
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