ArticleApplied and environmental microbiology2010
Expression of Xylella fastidiosa fimbrial and afimbrial proteins during biofilm formation.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Natural competence in the bacterial pathogen Xylella fastidiosa varies across genotypes and is associated with adhesins.PLoS pathogens · 2025Article
- XadA-like adhesin XADA2 regulates biofilm formation in X. fastidiosa subsp. fastidiosa putatively by engaging oleic-acid derived oxylipins.Molecular biology reports · 2025Article
- Genomic and phenotypic insight intoFrontiers in microbiology · 2023Article
- Comparative Genomics ofMicroorganisms · 2022Article
- Csp1, a Cold Shock Protein Homolog in Xylella fastidiosa Influences Cell Attachment, Pili Formation, and Gene Expression.Microbiology spectrum · 2021Article
- Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion.Biophysical journal · 2021Article
- Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits ofMicroorganisms · 2020Article
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- Ribosome Display Technology: Applications in Disease Diagnosis and Control.Antibodies (Basel, Switzerland) · 2020Review
- Biofilm architecture: An emerging synthetic biology target.Synthetic and systems biotechnology · 2020Review
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- Analyses of Seven New Genomes ofFrontiers in microbiology · 2019Article
- A Short Protocol for Gene Knockout and Complementation in Xylella fastidiosa Shows that One of the Type IV Pilin Paralogs (PD1926) Is Needed for Twitching while Another (PD1924) Affects Pilus Number and Location.Applied and environmental microbiology · 2018Article
- PAMPs, PRRs, effectors and R-genes associated with citrus-pathogen interactions.Annals of botany · 2017Review
- The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri.BMC microbiology · 2016Article
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- The Antitoxin Protein of a Toxin-Antitoxin System fromFrontiers in microbiology · 2016Article
- Spatiotemporal distribution of different extracellular polymeric substances and filamentation mediate Xylella fastidiosa adhesion and biofilm formation.Scientific reports · 2015Article
- Potential complications when developing gene deletion clones in Xylella fastidiosa.BMC research notes · 2015Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complete sequencing of the Xylella fastidiosa genome revealed characteristics that have not been described previously for a phytopathogen. One characteristic of this genome was the abundance of genes encoding proteins with adhesion functions related to biofilm formation, an essential step for colonization of a plant host or an insect vector. We examined four of the proteins belonging to this class encoded by genes in the genome of X. fastidiosa: the PilA2 and PilC fimbrial proteins, which are components of the type IV pili, and XadA1 and XadA2, which are afimbrial adhesins. Polyclonal antibodies were raised against these four proteins, and their behavior during biofilm development was assessed by Western blotting and immunofluorescence assays. In addition, immunogold electron microscopy was used to detect these proteins in bacteria present in xylem vessels of three different hosts (citrus, periwinkle, and hibiscus). We verified that these proteins are present in X. fastidiosa biofilms but have differential regulation since the amounts varied temporally during biofilm formation, as well as spatially within the biofilms. The proteins were also detected in bacteria colonizing the xylem vessels of infected plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.