ArticleGenome research2010
Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing.
Article in Genome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Mutagenesis ofFrontiers in plant science · 2020Article
- The BarleyFrontiers in genetics · 2020Article
- Article
- Targeted capture in evolutionary and ecological genomics.Molecular ecology · 2016Review
- Using next-generation sequencing to isolate mutant genes from forward genetic screens.Nature reviews. Genetics · 2014Review
- Forward genetics by genome sequencing reveals that rapid cyanide release deters insect herbivory of Sorghum bicolor.Genetics · 2013Article
- Organ-specific gene expression: the bHLH protein Sage provides tissue specificity to Drosophila FoxA.Development (Cambridge, England) · 2013Article
- Whole Genome Sequencing and a New Bioinformatics Platform Allow for Rapid Gene Identification in D. melanogaster EMS Screens.Biology · 2012Article
- Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing.BMC genomics · 2012Article
- A diploid wheat TILLING resource for wheat functional genomics.BMC plant biology · 2012Article
- Library-free methylation sequencing with bisulfite padlock probes.Nature methods · 2012Article
- Using Drosophila melanogaster as a Model for Genotoxic Chemical Mutational Studies with a New Program, SnpSift.Frontiers in genetics · 2012Article
- Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2012Article
- Article
- Exome-wide DNA capture and next generation sequencing in domestic and wild species.BMC genomics · 2011Article
- Multiplexed shotgun genotyping for rapid and efficient genetic mapping.Genome research · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
One of the key advantages of using Drosophila melanogaster as a genetic model organism is the ability to conduct saturation mutagenesis screens to identify genes and pathways underlying a given phenotype. Despite the large number of genetic tools developed to facilitate downstream cloning of mutations obtained from such screens, the current procedure remains labor intensive, time consuming, and costly. To address this issue, we designed an efficient strategy for rapid identification of heterozygous mutations in the fly genome by combining rough genetic mapping, targeted DNA capture, and second generation sequencing technology. We first tested this method on heterozygous flies carrying either a previously characterized dac(5) or sens(E2) mutation. Targeted amplification of genomic regions near these two loci was used to enrich DNA for sequencing, and both point mutations were successfully identified. When this method was applied to uncharacterized twr mutant flies, the underlying mutation was identified as a single-base mutation in the gene Spase18-21. This targeted-genome-sequencing method reduces time and effort required for mutation cloning by up to 80% compared with the current approach and lowers the cost to <$1000 for each mutant. Introduction of this and other sequencing-based methods for mutation cloning will enable broader usage of forward genetics screens and have significant impacts in the field of model organisms such as Drosophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.