ArticleThe Journal of biological chemistry2010
Identification of an ABCB/P-glycoprotein-specific inhibitor of auxin transport by chemical genomics.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 119 citations in OpenAlex.
- Chemical priming of hypoxia responses via PLANT CYSTEINE OXIDASE inhibition improves flooding tolerance.Plant physiology · 2026Article
- HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.Nature communications · 2025Article
- Conformational cycle and small-molecule inhibition mechanism of a plant ABCB transporter in lipid membranes.Science advances · 2025Article
- A key residue of the extracellular gate provides quality control contributing to ABCG substrate specificity.Nature communications · 2025Article
- Gravacin as an inhibitor of the auxin transport-activating protein kinase D6PK inFrontiers in plant science · 2025Article
- Plastid-localized amino acid metabolism coordinates rice ammonium tolerance and nitrogen use efficiency.Nature plants · 2023Article
- Auxin- and pH-induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth.Protoplasma · 2023Article
- Probing the Allosteric Modulation of P-Glycoprotein: A Medicinal Chemistry Approach Toward the Identification of Noncompetitive P-Gp Inhibitors.ACS omega · 2023Article
- Opposing effects ofPlant direct · 2022Article
- Systems approaches reveal that ABCB and PIN proteins mediate co-dependent auxin efflux.The Plant cell · 2022Article
- Loss of Multiple ABCB Auxin Transporters Recapitulates the MajorFrontiers in plant science · 2022Article
- Exploration of the Mechanisms of Differential Indole Alkaloid Biosynthesis in Dedifferentiated and Cambial Meristematic Cells ofFrontiers in genetics · 2022Article
- Electrophysiological study of Arabidopsis ABCB4 and PIN2 auxin transporters: Evidence of auxin activation and interaction enhancing auxin selectivity.Plant direct · 2021Article
- Chemical Biology in Auxin Research.Cold Spring Harbor perspectives in biology · 2021Review
- Naphthylphthalamic acid associates with and inhibits PIN auxin transporters.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Flavonol-mediated stabilization of PIN efflux complexes regulates polar auxin transport.The EMBO journal · 2021Article
- A Retro-Perspective on Auxin Transport.Frontiers in plant science · 2021Article
- The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes.Plant communications · 2020Article
- Auxin-transporting ABC transporters are defined by a conserved D/E-P motif regulated by a prolylisomerase.The Journal of biological chemistry · 2020Article
- Nyctinastic thallus movement in the liverwort Marchantia polymorpha is regulated by a circadian clock.Scientific reports · 2020Article
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Authors and funding
10 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant development and physiology are widely determined by the polar transport of the signaling molecule auxin. This process is controlled on the cellular efflux level catalyzed by members of the PIN (pin-formed) and ABCB (ATP-binding cassette protein subfamily B)/P-glycoprotein family that can function independently and coordinately. In this study, we have identified by means of chemical genomics a novel auxin transport inhibitor (ATI), BUM (2-[4-(diethylamino)-2-hydroxybenzoyl]benzoic acid), that efficiently blocks auxin-regulated plant physiology and development. In many respects, BUM resembles the functionality of the diagnostic ATI, 1-N-naphtylphtalamic acid (NPA), but it has an IC(50) value that is roughly a factor 30 lower. Physiological analysis and binding assays identified ABCBs, primarily ABCB1, as key targets of BUM and NPA, whereas PIN proteins are apparently not directly affected. BUM is complementary to NPA by having distinct ABCB target spectra and impacts on basipetal polar auxin transport in the shoot and root. In comparison with the recently identified ATI, gravacin, it lacks interference with ABCB membrane trafficking. Individual modes or targets of action compared with NPA are reflected by apically shifted root influx maxima that might be the result of altered BUM binding preferences or affinities to the ABCB nucleotide binding folds. This qualifies BUM as a valuable tool for auxin research, allowing differentiation between ABCB- and PIN-mediated efflux systems. Besides its obvious application as a powerful weed herbicide, BUM is a bona fide human ABCB inhibitor with the potential to restrict multidrug resistance during chemotherapy.
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