ArticleUrology2010
Effects of birth trauma and estrogen on urethral elastic fibers and elastin expression.
Article in Urology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Cytokine modulation in pelvic organ prolapse and urinary incontinence: from molecular insights to therapeutic targets.Molecular medicine (Cambridge, Mass.) · 2024Review
- Advances in the molecular pathogenesis and cell therapy of stress urinary incontinence.Frontiers in cell and developmental biology · 2023Review
- Article
- Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum.BMC musculoskeletal disorders · 2014Article
- Estrogen inhibits vaginal tropoelastin and TGF-β1 production.International urogynecology journal · 2012Article
- Pathology of urethral fibromuscular system related to parturition-induced stress urinary incontinence and TGF-β1/Smad pathway.Molecular and cellular biochemistry · 2012Article
- TGF-beta 1 is a potential regulator of vaginal tropoelastin production.International urogynecology journal · 2012Article
- Effects of prolonged vaginal distension and β-aminopropionitrile on urinary continence and urethral structure.Urology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectivesTo investigate the effects of birth trauma and estrogen on urethral elastic fibers and elastin expression.
methodsPregnant rats were subjected to sham operation (Delivery-only), DVDO (delivery, vaginal distension and ovariectomy), or DVDO + E₂ (estrogen). At 2, 4, 8, or 12 weeks, their urethras were harvested for elastic fiber staining and reverse transcription-polymerase chain reaction analysis. Urethral cells were treated with transforming growth factor- β1 (TGFβ1) and/or estrogen and analyzed for elastin mRNA expression. Urethral cells were also examined for the activities of Smad1- and Smad3/4-responsive elements in response to TGFβ1 and estrogen.
resultsAt 8 weeks post-treatment, the urethras of DVDO rats had fewer and shorter elastic fibers when compared with Delivery-only rats, and those of DVDO + E₂ rats had fewer and shorter elastic fibers when compared with DVDO rats. Elastin mRNA was expressed at low levels in Delivery-only rats and at increasingly higher levels in DVDO rats at 2, 4, and 8 weeks but at sharply lower levels in DVDO + E₂ rats when compared with DVDO rats at 8 weeks. Urethral cells expressed increasingly higher levels of elastin mRNA in response to increasing concentrations of TGFβ1 up to 1 ng/mL. At this TGFβ1 concentration, urethral cells expressed significantly lower levels of elastin mRNA when treated with estrogen before or after TGFβ1 treatment. Both Smad1- and Smad3/4-responsive elements were activated by TGFβ1 and such activation was suppressed by estrogen.
conclusionsBirth trauma appears to activate urethral elastin expression via TGFβ1 signaling. Estrogen interferes with this signaling, resulting in improper assembly of elastic fibers.
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Registered trials
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