ArticleBMC infectious diseases2010
Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR.
Article in BMC infectious diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Zoonotic Mycobacterium bovis-induced tuberculosis in humans.Emerging infectious diseases · 2013Pooled it
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- Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad.BioMed research international · 2014Trial
- Direct detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis.PLoS neglected tropical diseases · 2021Article
- Molecular typing and drug sensitivity testing of Mycobacterium tuberculosis isolated by a community-based survey in Ethiopia.BMC public health · 2015Article
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Characterization of mycobacterium isolates from pulmomary tuberculosis suspected cases visiting Tuberculosis Reference Laboratory at Ethiopian Health and Nutrition Research Institute, Addis Ababa Ethiopia: a cross sectional study.Asian Pacific journal of tropical medicine · 2015Article
- Article
- Rapid detection of MDR-Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens.Asian Pacific journal of tropical biomedicine · 2014Article
- Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex.BMC infectious diseases · 2014Article
- Comparison of four DNA extraction methods for detecting Mycobacterium tuberculosis by real-time PCR and its clinical application in pulmonary tuberculosis.Journal of thoracic disease · 2013Article
- Article
- Mycobacterium bovis infection at the interface between domestic and wild animals in Zambia.BMC veterinary research · 2012Article
- Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolated in Nepal.Antimicrobial agents and chemotherapy · 2012Article
- Mycobacterial infection after intravesical bacillus Calmette-Guërin treatment for bladder cancer: a case report.The Korean journal of laboratory medicine · 2011Article
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7 authors.
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Abstract
backgroundSpecies identification of isolates belonging to the Mycobacterium tuberculosis complex (MTC) seems to be important for the appropriate treatment of patients, since M. bovis is naturally resistant to a first line anti-tuberculosis (TB) drug, pyrazinamide, while most of the other MTC members are susceptible to this antimicrobial agent. A simple and low-cost differentiation method was needed in higher TB burden countries, such as Bangladesh, where the prevalence of M. bovis among people or cattle has not been investigated.
methodsGenetic regions cfp32, RD9 and RD12 were chosen as targets for a species distinguishable multiplex PCR and the system was evaluated with twenty reference strains of mycobacterial species including non-tubercular mycobacteria (NTM). A total of 350 clinical MTC isolates obtained in Bangladesh were then analyzed with this multiplex PCR.
resultsAll of the MTC reference strains gave expected banding patterns and no non-specific amplifications were observed in the NTM strains. Out of 350 clinical isolates examined by this method, 347 (99.1%) were positive for all of the cfp32, RD9 and RD12 and determined as M. tuberculosis. Two isolates lacked cfp32 PCR product and one lacked RD12, however, those three samples were further examined and identified as M. tuberculosis by the sequence analyses of hsp65 and gyrB.
conclusionsThe MTC-discrimination multiplex PCR (MTCD-MPCR) developed in this study showed high specificity and was thought to be very useful as a routine test because of its simplicity. In the current survey, all the 350 MTC isolates obtained from Bangladesh TB patients were determined as M. tuberculosis and no other MTC were detected. This result suggested the general TB treatment regimen including pyrazinamide to be the first choice in Bangladesh.
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