ArticleLipids2010
Identification and characterization of Delta12, Delta6, and Delta5 Desaturases from the green microalga Parietochloris incisa.
Article in Lipids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 47 citations in OpenAlex.
- Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red MicroalgaMarine drugs · 2024Article
- Synthesis of CInternational journal of molecular sciences · 2022Review
- Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use.Applied microbiology and biotechnology · 2020Review
- Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich MicroalgaFrontiers in plant science · 2020Article
- Molecular cloning and functional analysis of a Δ3 Biotech · 2019Article
- Functional characterization and substrate specificity analysis of Δ6-desaturase from marine microalga Isochrysis sp.Biotechnology letters · 2018Article
- An Evolutionary Perspective on Linoleic Acid Synthesis in Animals.Evolutionary biology · 2018Article
- Molecular mechanisms underlying catalytic activity of delta 6 desaturase fromJournal of lipid research · 2018Article
- Molecular mechanism of substrate specificity for delta 6 desaturase from Mortierella alpina and Micromonas pusilla.Journal of lipid research · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The freshwater microalga Parietochloris incisa accumulates, under nitrogen starvation, large amounts of triacylglycerols containing approximately 60% of the omega6 very long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid. Based on sequence homology, we isolated three cDNA sequences from P. incisa, designated PiDesD12, PiDesD6, PiDesD5. The deduced amino acid sequences of the three genes contained three conserved histidine motifs; the front-end desaturases, PiDes6 and PiDes5, contained a fused N-terminal cytochrome b5 domain. By functional characterization in the yeast Saccharomyces cerevisiae, we confirmed that PiDesD6, PiDesD5 cDNA encode membrane bound desaturases with Delta6, and Delta5 activity, respectively. Both PiDes6 and PiDes5 can indiscriminately desaturate both omega6 and omega3 substrates. A phylogenetic analysis showed that the three genes were homologous to the corresponding desaturases from green microalgae and lower plants that were functionally characterized. Quantitative real-time PCR revealed the concerted expression pattern of all three genes in P. incisa cells subjected to nitrogen starvation, featuring maximum expression level on day 3 of starvation, corresponding to the sharpest increase in the share of arachidonic acid.
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