ArticleMolecular & cellular proteomics : MCP2011
Loss of Dicer in Sertoli cells has a major impact on the testicular proteome of mice.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 97 citations in OpenAlex.
- MicroRNAs in seminal plasma are able to discern infertile men at increased risk of developing testicular cancer.Molecular oncology · 2025Article
- Exploring the Characters of Non-Coding RNAs in Spermatogenesis and Male Infertility.International journal of molecular sciences · 2025Review
- Inhibition ofCells · 2024Article
- Follicle-stimulating hormone-mediated decline in miR-92a-3p expression in pubertal mice Sertoli cells is crucial for germ cell differentiation and fertility.Cellular and molecular life sciences : CMLS · 2022Article
- Regulation of mammalian spermatogenesis by miRNAs.Seminars in cell & developmental biology · 2022Review
- Comparison of miRNA and mRNA Expression in Sika Deer Testes With Age.Frontiers in veterinary science · 2022Article
- Declining levels of miR-382-3p at puberty trigger the onset of spermatogenesis.Molecular therapy. Nucleic acids · 2021Article
- Micro RNAs in Regulation of Cellular Redox Homeostasis.International journal of molecular sciences · 2021Review
- Epididymal epithelium propels early sexual transmission of Zika virus in the absence of interferon signaling.Nature communications · 2021Article
- The Roles of MicroRNAs in Male Infertility.International journal of molecular sciences · 2021Review
- Distinct expression patterns of seven crucial microRNAs during early embryonic development in medaka (Oryzias latipes).Gene expression patterns : GEP · 2020Article
- miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting theFrontiers in genetics · 2020Article
- Profiling of miRNAs in porcine Sertoli cells.Journal of animal science and biotechnology · 2020Article
- miR-202-3p Regulates Sertoli Cell Proliferation, Synthesis Function, and Apoptosis by Targeting LRP6 and Cyclin D1 of Wnt/β-Catenin Signaling.Molecular therapy. Nucleic acids · 2019Article
- MicroRNAs: Recent insights towards their role in male infertility and reproductive cancers.Bosnian journal of basic medical sciences · 2019Review
- Routes of Zika virus dissemination in the testis and epididymis of immunodeficient mice.Nature communications · 2018Article
- MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation.Scientific reports · 2018Article
- Decreased AGO2 and DCR1 in PBMCs from War Veterans with PTSD leads to diminished miRNA resulting in elevated inflammation.Translational psychiatry · 2017Article
- Dynamics of miRNA transcriptome during gonadal development of zebrafish.Scientific reports · 2017Article
- Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer.Scientific reports · 2017Article
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sertoli cells (SCs) are the central, essential coordinators of spermatogenesis, without which germ cell development cannot occur. We previously showed that Dicer, an RNaseIII endonuclease required for microRNA (miRNA) biogenesis, is absolutely essential for Sertoli cells to mature, survive, and ultimately sustain germ cell development. Here, using isotope-coded protein labeling, a technique for protein relative quantification by mass spectrometry, we investigated the impact of Sertoli cell-Dicer and subsequent miRNA loss on the testicular proteome. We found that, a large proportion of proteins (50 out of 130) are up-regulated by more that 1.3-fold in testes lacking Sertoli cell-Dicer, yet that this protein up-regulation is mild, never exceeding a 2-fold change, and is not preceeded by alterations of the corresponding mRNAs. Of note, the expression levels of six proteins of interest were further validated using the Absolute Quantification (AQUA) peptide technology. Furthermore, through 3'UTR luciferase assays we identified one up-regulated protein, SOD-1, a Cu/Zn superoxide dismutase whose overexpression has been linked to enhanced cell death through apoptosis, as a likely direct target of three Sertoli cell-expressed miRNAs, miR-125a-3p, miR-872 and miR-24. Altogether, our study, which is one of the few in vivo analyses of miRNA effects on protein output, suggests that, at least in our system, miRNAs play a significant role in translation control.
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