ArticleMolecular & cellular proteomics : MCP2010
Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Quantitative proteomic analysis based on TMT reveals different responses of Haloxylon ammodendron and Haloxylon persicum to long-term drought.BMC plant biology · 2025Article
- CRKL but not CRKII contributes to hemin-induced erythroid differentiation of CML.Journal of cellular and molecular medicine · 2024Article
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- Comparative Analysis of Global Proteome and Lysine Acetylome Between Naive CD4Frontiers in immunology · 2021Article
- ALK4 coordinates extracellular and intrinsic signals to regulate development of cortical somatostatin interneurons.The Journal of cell biology · 2020Article
- Serum-Based Proteomics Reveals Lipid Metabolic and Immunoregulatory Dysregulation in Cervical Artery Dissection With Stroke.Frontiers in neurology · 2020Article
- Metabo groups in response to micronutrient intervention: Pilot study.Food science & nutrition · 2020Article
- iTRAQ-based quantitative proteomic and physiological analysis of the response to N deficiency and the compensation effect in rice.BMC genomics · 2019Article
- TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.Frontiers in immunology · 2019Article
- Quantitative proteomic characterization and comparison of T helper 17 and induced regulatory T cells.PLoS biology · 2018Article
- Significant and unique changes in phosphorylation levels of four phosphoproteins in two apple rootstock genotypes under drought stress.Molecular genetics and genomics : MGG · 2017Article
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- The Extrinsic Coagulation Pathway: a Biomarker for Suicidal Behavior in Major Depressive Disorder.Scientific reports · 2016Article
- Insights into the adaptive response of the plant-pathogenic oomycete Phytophthora capsici to the fungicide flumorph.Scientific reports · 2016Article
- Quantitative Proteomics of Gut-Derived Th1 and Th1/Th17 Clones Reveal the Presence of CD28+ NKG2D- Th1 Cytotoxic CD4+ T cells.Molecular & cellular proteomics : MCP · 2016Article
- Electroacupuncture pretreatment exhibits anti-depressive effects by regulating hippocampal proteomics in rats with chronic restraint stress.Neural regeneration research · 2015Article
- A Combined Omics Approach to Generate the Surface Atlas of Human Naive CD4+ T Cells during Early T-Cell Receptor Activation.Molecular & cellular proteomics : MCP · 2015Article
- Combined Metabolomics and Proteomics Analysis of Major Depression in an Animal Model: Perturbed Energy Metabolism in the Chronic Mild Stressed Rat Cerebellum.Omics : a journal of integrative biology · 2015Article
- From a gene-centric to whole-proteome view of differentiation of T helper cell subsets.Briefings in functional genomics · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4(+) cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4(+) T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.
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