ArticleThe Journal of molecular diagnostics : JMD2010
Comparison of Shiga toxin-producing Escherichia coli detection methods using clinical stool samples.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Characterization of Broad Spectrum Bacteriophage vB ESM-pEJ01 and Its Antimicrobial Efficacy Against Shiga Toxin-ProducingMicroorganisms · 2025Article
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- Infectious agents present in monk parakeet (Frontiers in veterinary science · 2023Article
- Virulence Determinants and Multidrug Resistance ofAntibiotics (Basel, Switzerland) · 2021Article
- Molecular Detection of Avian PathogenicMicroorganisms · 2020Article
- Incidence, Pathotyping, and Antibiotic Susceptibility of Avian PathogenicPathogens (Basel, Switzerland) · 2020Article
- Contribution and Interaction of Shiga Toxin Genes toToxins · 2019Article
- Direct-from-Stool Testing for Shiga Toxins by an Immunochromatographic Assay Is Insensitive for Detection of Shigatoxigenic Escherichia coli.Journal of clinical microbiology · 2018Article
- Pooled Nucleic Acid Amplification Test for Screening of Stool Specimens for Shiga Toxin-Producing Escherichia coli.Journal of clinical microbiology · 2016Article
- Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants.Applied and environmental microbiology · 2016Article
- Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Review
- Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada.Journal of clinical microbiology · 2015Article
- Article
- A sensitive multiplex, real-time PCR assay for prospective detection of Shiga toxin-producing Escherichia coli from stool samples reveals similar incidences but variable severities of non-O157 and O157 infections in northern California.Journal of clinical microbiology · 2013Article
- Comparison of three different methods for detection of Shiga toxin-producing Escherichia coli in a tertiary pediatric care center.Journal of clinical microbiology · 2013Article
- Escherichia coli O104:H4 infections and international travel.Emerging infectious diseases · 2012Article
- Prevalence of shiga toxin-producing Escherichia coli as detected by enzyme-linked immunoassays and real-time PCR during the summer months in northern Alberta, Canada.Journal of clinical microbiology · 2011Article
- Shiga-toxigenic Escherichia coli detection in stool samples screened for viral gastroenteritis in Alberta, Canada.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular diagnostic tools capable of identifying Shiga toxin-specific genetic determinants in stool specimens permit an unbiased approach to detect Shiga toxin-producing Escherichia coli (STEC) in clinical samples and can indicate when culture-based isolation methods are required. It is increasingly recognized that clinically relevant STEC are not limited to the singular O157 serotypes, and therefore diagnostic assays targeting toxin-encoding determinants must be able to account for any genetic variation that exists between serotypes. In this study conventional PCR and four real-time PCR assays (HybProbe, TaqMan, SYBR Green, and LUX) targeting the stx1 and stx2 Shiga toxin coding sequences were used to identify STEC in enriched stool samples (n = 36) and a panel of O157 and non-O157 strains (n = 64). PCR assays targeting stx1 and stx2 had variable specificity and sensitivity values with enriched stool samples. Molecular assays using DNA from pure cultures revealed that some primers were not sensitive to all stx2 variants. This evaluation concluded that the TaqMan-based probes were most appropriate in high throughput clinical diagnostic laboratories in consideration of cost, turn around time, and assay performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.