ArticleNucleic acids research2010
Structure of the K-turn U4 RNA: a combined NMR and SANS study.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The Kink-Turn 7 Motif: An Additional Test for RNA Force Field Performance.Journal of chemical theory and computation · 2025Article
- Discovery of small molecule probes targeting the 5' stem-loop in the yeast U4/U6 snRNA.bioRxiv : the preprint server for biology · 2025Article
- Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites.Scientific reports · 2021Article
- Identification of small molecules that mitigate vincristine-induced neurotoxicity while sensitizing leukemia cells to vincristine.Clinical and translational science · 2021Article
- Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.Chemical science · 2020Article
- Computational Investigation of RNA A-Bulges Related to the Microtubule-Associated Protein Tau Causing Frontotemporal Dementia and Parkinsonism.The journal of physical chemistry. B · 2019Article
- RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.Chemical reviews · 2018Review
- Alteration of RNA Splicing by Small-Molecule Inhibitors of the Interaction between NHP2L1 and U4.SLAS discovery : advancing life sciences R & D · 2018Article
- NMR experiments for the rapid identification of P=O···H-X type hydrogen bonds in nucleic acids.Journal of biomolecular NMR · 2017Article
- Applications of NMR to structure determination of RNAs large and small.Archives of biochemistry and biophysics · 2017Review
- Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA.Journal of molecular biology · 2016Article
- RNA structure determination by solid-state NMR spectroscopy.Nature communications · 2015Article
- The dynamic duo: combining NMR and small angle scattering in structural biology.Protein science : a publication of the Protein Society · 2014Review
- Interactions of noncanonical motifs with hnRNP A2 promote activity-dependent RNA transport in neurons.The Journal of cell biology · 2014Article
- Elucidating molecular motion through structural and dynamic filters of energy-minimized conformer ensembles.The journal of physical chemistry. B · 2014Article
- Small molecule binding, docking, and characterization of the interaction between Pth1 and peptidyl-tRNA.International journal of molecular sciences · 2013Article
- Review
- Theory of nonrigid rotational motion applied to NMR relaxation in RNA.The journal of physical chemistry. A · 2011Article
- Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31.RNA (New York, N.Y.) · 2011Article
- Nuclear magnetic resonance analysis of protein-DNA interactions.Journal of the Royal Society, Interface · 2011Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
K-turn motifs are universal RNA structural elements providing a binding platform for proteins in several cellular contexts. Their characteristic is a sharp kink in the phosphate backbone that puts the two helical stems of the protein-bound RNA at an angle of 60°. However, to date no high-resolution structure of a naked K-turn motif is available. Here, we present the first structural investigation at atomic resolution of an unbound K-turn RNA (the spliceosomal U4-Kt RNA) by a combination of NMR and small-angle neutron scattering data. With this study, we wish to address the question whether the K-turn structural motif assumes the sharply kinked conformation in the absence of protein binders and divalent cations. Previous studies have addressed this question by fluorescence resonance energy transfer, biochemical assays and molecular dynamics simulations, suggesting that the K-turn RNAs exist in equilibrium between a kinked conformation, which is competent for protein binding, and a more extended conformation, with the population distribution depending on the concentration of divalent cations. Our data shows that the U4-Kt RNA predominantly assumes the more extended conformation in the absence of proteins and divalent cations. The internal loop region is well structured but adopts a different conformation from the one observed in complex with proteins. Our data suggests that the K-turn consensus sequence does not per se code for the kinked conformation; instead the sharp backbone kink requires to be stabilized by protein binders.
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