ArticleMolecular and cellular biology1991
The myosin light chain enhancer and the skeletal actin promoter share a binding site for factors involved in muscle-specific gene expression.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Cell-specific transcription of the smooth muscle gamma-actin gene requires both positive- and negative-acting cis elements.Gene expression · 1998Article
- Growth and differentiation of C2 myogenic cells are dependent on serum response factor.Molecular and cellular biology · 1996Article
- Expression and activity of serum response factor is required for expression of the muscle-determining factor MyoD in both dividing and differentiating mouse C2C12 myoblasts.Molecular biology of the cell · 1996Article
- Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle.Molecular and cellular biology · 1995Article
- Activation of a muscle-specific enhancer by the Ski proto-oncogene.Nucleic acids research · 1995Article
- Regulation of a muscle-specific transgene by retinoic acid.The Journal of cell biology · 1995Article
- Developmental stage-specific regulation of atrial natriuretic factor gene transcription in cardiac cells.Molecular and cellular biology · 1994Article
- cis-acting sequences of the rat troponin I slow gene confer tissue- and development-specific transcription in cultured muscle cells as well as fiber type specificity in transgenic mice.Molecular and cellular biology · 1993Article
- Analysis of the rabbit cardiac/slow twitch muscle sarcoplasmic reticulum calcium ATPase (SERCA2) gene promoter.Nucleic acids research · 1993Article
- Multiple regulatory elements contribute differentially to muscle creatine kinase enhancer activity in skeletal and cardiac muscle.Molecular and cellular biology · 1993Article
- Serum response factor p67SRF is expressed and required during myogenic differentiation of both mouse C2 and rat L6 muscle cell lines.The Journal of cell biology · 1992Article
- Natural and synthetic DNA elements with the CArG motif differ in expression and protein-binding properties.Molecular and cellular biology · 1991Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The myosin light chain (MLC) 1/3 enhancer (MLC enhancer), identified at the 3' end of the skeletal MLC1/3 locus, contains a sequence motif that is homologous to a protein-binding site of the skeletal muscle alpha-actin promoter. Gel shift, competition, and footprint assays demonstrated that a CArG motif in the MLC enhancer binds the proteins MAPF1 and MAPF2, previously identified as factors interacting with the muscle regulatory element of the skeletal alpha-actin promoter. Transient transfection assays with constructs containing the chloramphenicol acetyltransferase reporter gene demonstrated that a 115-bp subfragment of the MLC enhancer is able to exert promoter activity when provided with a silent nonmuscle TATA box. A point mutation at the MAPF1/2-binding site interferes with factor binding and abolishes the promoter activity of the 115-bp fragment. The observation that an oligonucleotide encompassing the MAPF1/2 site of the MLC enhancer alone cannot serve as a promoter element suggests that additional factor-binding sites are necessary for this function. The finding that MAPF1 and MAPF2 recognize similar sequence motifs in two muscle genes, simultaneously activated during muscle differentiation, implies that these factors may have a role in coordinating the activation of contractile protein gene expression during myogenesis.
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