ArticleMolecular and cellular biology1991
Pituitary pro-opiomelanocortin gene expression requires synergistic interactions of several regulatory elements.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 116 citations in OpenAlex.
- Nuclear Receptors as Regulators of Pituitary Corticotroph Pro-Opiomelanocortin Transcription.Cells · 2020Review
- Pituitary cell translation and secretory capacities are enhanced cell autonomously by the transcription factor Creb3l2.Nature communications · 2019Article
- Inhibitory Effects of a Novel PPAR-PPAR research · 2018Article
- Article
- The Ets factor Etv1 interacts with Tpit protein for pituitary pro-opiomelanocortin (POMC) gene transcription.The Journal of biological chemistry · 2011Article
- The human POMC gene promoter: where do we stand?Journal of endocrinological investigation · 2011Review
- A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Deducing corticotropin-releasing hormone receptor type 1 signaling networks from gene expression data by usage of genetic algorithms and graphical Gaussian models.BMC systems biology · 2010Article
- Hypothalamic proopiomelanocortin promoter methylation becomes altered by early overfeeding: an epigenetic model of obesity and the metabolic syndrome.The Journal of physiology · 2009Article
- Pro-opiomelanocortin gene variation related to alcohol or drug dependence: evidence and replications across family- and population-based studies.Biological psychiatry · 2009Article
- Potential of retinoic acid derivatives for the treatment of corticotroph pituitary adenomas.Reviews in endocrine & metabolic disorders · 2009Review
- TIF1beta/KAP-1 is a coactivator of the orphan nuclear receptor NGFI-B/Nur77.The Journal of biological chemistry · 2009Article
- Single nucleotide variants of the TGACTCA motif modulate energetics and orientation of binding of the Jun-Fos heterodimeric transcription factor.Biochemistry · 2009Article
- A composite element that binds basic helix loop helix and basic leucine zipper transcription factors is important for gonadotropin-releasing hormone regulation of the follicle-stimulating hormone beta gene.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Developmental dependence on NurRE and EboxNeuro for expression of pituitary proopiomelanocortin.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Dimer-specific potentiation of NGFI-B (Nur77) transcriptional activity by the protein kinase A pathway and AF-1-dependent coactivator recruitment.Molecular and cellular biology · 2003Article
- Retinoic acid prevents experimental Cushing syndrome.The Journal of clinical investigation · 2001Article
- Specific protein-protein interaction between basic helix-loop-helix transcription factors and homeoproteins of the Pitx family.Molecular and cellular biology · 2000Article
- Heterodimerization between members of the Nur subfamily of orphan nuclear receptors as a novel mechanism for gene activation.Molecular and cellular biology · 1999Article
- Ptx1 regulates SF-1 activity by an interaction that mimics the role of the ligand-binding domain.The EMBO journal · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pro-opiomelanocortin (POMC) gene is expressed very early during pituitary development, before expression of the other pituitary hormone genes, growth hormone and prolactin, and before expression of the Pit-1/GHF-1 transcription factor which activates those genes. Thus, analysis of the POMC promoter should provide markers of the early stages of pituitary development at the time when cells are being committed to expression of one or the other pituitary hormone. We have previously localized the rat POMC promoter to a 543-bp 5'-flanking DNA fragment of the gene using transfection and transgenic mice experiments. We have now used mutagenesis and in vitro protein-DNA binding studies to define three domains of the promoter which have distinct and complementary activities. Within these domains which require each other for full activity, at least nine regulatory elements were defined by in vitro footprinting and replacement mutagenesis. Each element appeared equally important for promoter activity, as mutagenesis of any element had similar effect on promoter activity. Most of the elements bound different AtT-20 nuclear proteins in gel mobility shift experiments. Whereas only two elements appeared to be binding sites for the known transcription factors AP-1 and chicken ovalbumin upstream promoter, the seven other elements appeared to bind nuclear proteins with novel properties. Thus, in contrast to the predominant role of Pit-1/GHF-1 in transcription of the growth hormone and prolactin genes, the control of an early pituitary gene, POMC, appears to depend on the synergistic interaction of several regulatory elements which bind different nuclear proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.