ArticleActa biomaterialia2010
Proteomic evaluation of biological nanoparticles isolated from human kidney stones and calcified arteries.
Article in Acta biomaterialia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Clinical implications of calcifying nanoparticles in dental diseases: a critical review.International journal of nanomedicine · 2014Pooled it
- Preliminary study of the role of nanobacteria in the formation of renal stones in experimental rats and its mechanism.Archives of medical sciences. Atherosclerotic diseases · 2024Article
- Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation.Scientific reports · 2017Article
- Article
- Calcifying nanoparticles promote mineralization in vascular smooth muscle cells: implications for atherosclerosis.International journal of nanomedicine · 2014Article
- Are calcifying microvesicles another analogous substructure of calcifying nanoparticles?International journal of nanomedicine · 2013Article
- Bions: a family of biomimetic mineralo-organic complexes derived from biological fluids.PloS one · 2013Article
- The role of calcifying nanoparticles in biology and medicine.International journal of nanomedicine · 2012Review
- Do calcifying nanoparticles really contain 16S rDNA?International journal of nanomedicine · 2012Article
- Association between calcifying nanoparticles and placental calcification.International journal of nanomedicine · 2012Article
- The association of matrix Gla protein isomers with calcification in capsules surrounding silicone breast implants.Biomaterials · 2011Article
- High-speed microscopic imaging of flagella motility and swimming in Giardia lamblia trophozoites.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro.Acta biomaterialia · 2011Article
- Nanobacteria in the pathogenesis of urolithiasis: Myth or reality?Indian journal of urology : IJU : journal of the Urological Society of IndiaReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Calcifying biological nanoparticles (NPs) develop under cell culture conditions from homogenates of diverse tissue samples displaying extraosseous mineralization, including kidney stones and calcified aneurysms. Probes to definitively identify NPs in biological systems are lacking. Therefore, the aim of this study was to begin to establish a proteomic biosignature of NPs in order to facilitate more definitive investigation of their contribution to disease. Biological NPs derived from human kidney stones and calcified aneurysms were completely decalcified by overnight treatment with ethylenediaminetetraacetic acid or brief incubation in HCl, as evidenced by lack of a calcium shell and of Alizarin Red S staining, by transmission electron microscopy and confocal microscopy, respectively. Decalcified NPs contained numerous proteins, including some from bovine serum and others of prokaryotic origin. Most prominent of the latter group was EF-Tu, which appeared to be identical to EF-Tu from Staphylococcus epidermidis. A monoclonal antibody against human EF-Tu recognized a protein in Western blots of total NP lysate, as well as in intact NPs by immunofluorescence and immunogold EM. Approximately 8% of NPs were quantitatively recognized by the antibody using flow cytometry. Therefore, we have defined methods to reproducibly decalcify biological NPs, and identified key components of their proteome. These elements, including EF-Tu, can be used as biomarkers to further define the processes that mediate propagation of biological NPs and their contribution to disease.
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