ReviewJournal of structural biology2010
Biophysical characterization of recombinant proteins: a key to higher structural genomics success.
Review in Journal of structural biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Article
- Understanding the strengths and limitations of recombinant protein studies.Frontiers in molecular biosciences · 2026Review
- Development of Peptide Displacement Assays to Screen for Antagonists of DDB1 Interactions.Biochemistry · 2024Article
- Heterologous Expression of Difficult to Produce Proteins in Bacterial Systems.International journal of molecular sciences · 2024Article
- Glutaryl-CoA Dehydrogenase Misfolding in Glutaric Acidemia Type 1.International journal of molecular sciences · 2023Article
- Recombinant Expression and Biophysical Characterization of a DruggableAdvanced pharmaceutical bulletin · 2022Article
- The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters.Nature protocols · 2021Review
- In-depth interrogation of protein thermal unfolding data with MoltenProt.Protein science : a publication of the Protein Society · 2021Article
- The Trp triad within the V-domain of the receptor for advanced glycation end products modulates folding, stability and ligand binding.Bioscience reports · 2020Article
- Optimization of Buffer Additives for Efficient Recovery of hGM-CSF from Inclusion Bodies Using Response Surface Methodology.Iranian journal of pharmaceutical research : IJPR · 2020Article
- Determining the Protein Stability of Alzheimer's Disease Protein, Amyloid Precursor Protein.The protein journal · 2019Article
- Selection of Biophysical Methods for Characterisation of Membrane Proteins.International journal of molecular sciences · 2019Review
- Principles for computational design of binding antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Exploring Covalent Allosteric Inhibition of Antigen 85C from Mycobacterium tuberculosis by Ebselen Derivatives.ACS infectious diseases · 2017Article
- Article
- Protein Crystallography in Vaccine Research and Development.International journal of molecular sciences · 2015Review
- A general protocol for the generation of Nanobodies for structural biology.Nature protocols · 2014Article
- Article
- Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization.Expert opinion on drug discovery · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have paved the way for structural genomics efforts. Frequently, however, little is known about newly expressed proteins calling for large-scale protein characterization to better understand their biochemical roles and to enable structure-function relationship studies. In the Structural Genomics Consortium (SGC), we have established a platform to characterize large numbers of purified proteins. This includes screening for ligands, enzyme assays, peptide arrays and peptide displacement in a 384-well format. In this review, we describe this platform in more detail and report on how our approach significantly increases the success rate for structure determination. Coupled with high-resolution X-ray crystallography and structure-guided methods, this platform can also be used toward the development of chemical probes through screening families of proteins against a variety of chemical series and focused chemical libraries.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.