Evidence map›Paper›PMID 20464356›Full record

ArticleHNO2010

[Impact of TGF-beta1 antisense on collagen-binding integrins in keloid].

G Bran, U Sommer, F Meinzer, U R Goessler, K Hörmann, F Riedel, H Sadick

Abstract readEnglish Abstract
PubMed Publisher
In one paragraph

Article in HNO, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

G BranUniversitäts-HNO-Klinik, Universitätsklinikum Mannheim, Universität Heidelberg, Deutschland. gregor.bran@umm.de
U Sommer
F Meinzer
U R Goessler
K Hörmann
F Riedel
H Sadick
Universitäts HNO Klinik Mannheim · DEHeidelberg University · DEUniversitäts-HNO-Klinik Heidelberg · DEUniversity Medical Centre Mannheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe treatment of keloids remains challenging due to sparse knowledge about the pathogenesis of this disease. Transforming growth factor (TGF)-beta1 plays a central role in keloid formation. Cell-matrix communication is controlled by integrins, the expression of which can be regulated by TGF-beta1.

methodsUsing immunohistochemistry we compared expression patterns of alpha1beta1, alpha2beta1 und alpha3beta1 in normal skin and keloid tissue. Secondly, the effect of TGF-beta1-antisense after 48 h and 72 h incubation in a keloid-derived fibroblast monolayer was analyzed by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry.

resultsalpha1beta1 and alpha2beta1 were highly expressed in keloid fibroblasts. Incubation with TGF-beta1-antisense lead to a reduction on protein level. RT-PCR demonstrated an increase of all alpha subunits, while on an mRNA level a decrease of the subunit beta1 could be observed.

conclusionIntegrin expression is directly modulated by TGF-beta1. An abnormal response in the keloid as a result of an altered TGF-beta1 pathway could be a key element to understanding the development of keloids.

Indexed as

CollagenFibroblastsHumansImmunoenzyme TechniquesIntegrinsKeloidOligonucleotides, AntisenseReverse Transcriptase Polymerase Chain ReactionSkinTransforming Growth Factor beta1CollagenIntegrinsOligonucleotides, AntisenseTGFB1 protein, humanTransforming Growth Factor beta1

Identifiers

PMID20464356
OpenAlexW1591484890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.