ArticlePloS one2010
Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 93 citations in OpenAlex.
- Using Phylogeny and a Conserved Genomic Neighborhood Analysis to Extract and Visualize Gene Sets Involved in Target Gene Function: The Case of [NiFe]-hydrogenase and Succinate Dehydrogenase.Microbes and environments · 2025Article
- Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations.Microbial cell factories · 2022Article
- Towards a Synthetic Biology Toolset for Metallocluster Enzymes in Biosynthetic Pathways: What We Know and What We Need.Molecules (Basel, Switzerland) · 2021Review
- Large-scale computational discovery and analysis of virus-derived microbial nanocompartments.Nature communications · 2021Article
- Protein Engineering of the Soluble Metal-dependent Formate Dehydrogenase from Escherichia coli.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.International journal of molecular sciences · 2020Review
- Genetic analyses of the functions of [NiFe]-hydrogenase maturation endopeptidases in the hyperthermophilic archaeon Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2017Article
- Activation of formate hydrogen-lyase via expression of uptake [NiFe]-hydrogenase in Escherichia coli BL21(DE3).Microbial cell factories · 2015Article
- The multifaceted benefits of protein co-expression in Escherichia coli.Journal of visualized experiments : JoVE · 2015Article
- Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus.Archaea (Vancouver, B.C.) · 2015Review
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.Applied and environmental microbiology · 2014Article
- Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon.Biotechnology for biofuels · 2013Article
- Dual organism design cycle reveals small subunit substitutions that improve [NiFe] hydrogenase hydrogen evolution.Journal of biological engineering · 2013Article
- Article
- Design and development of synthetic microbial platform cells for bioenergy.Frontiers in microbiology · 2013Article
- Extreme Thermophiles: Moving beyond single-enzyme biocatalysis.Current opinion in chemical engineering · 2012Article
- Hydrogen production by recombinant Escherichia coli strains.Microbial biotechnology · 2012Review
- Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase.The Journal of biological chemistry · 2012Article
- A synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolism.Journal of biological engineering · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen gas is a major biofuel and is metabolized by a wide range of microorganisms. Microbial hydrogen production is catalyzed by hydrogenase, an extremely complex, air-sensitive enzyme that utilizes a binuclear nickel-iron [NiFe] catalytic site. Production and engineering of recombinant [NiFe]-hydrogenases in a genetically-tractable organism, as with metalloprotein complexes in general, has met with limited success due to the elaborate maturation process that is required, primarily in the absence of oxygen, to assemble the catalytic center and functional enzyme. We report here the successful production in Escherichia coli of the recombinant form of a cytoplasmic, NADP-dependent hydrogenase from Pyrococcus furiosus, an anaerobic hyperthermophile. This was achieved using novel expression vectors for the co-expression of thirteen P. furiosus genes (four structural genes encoding the hydrogenase and nine encoding maturation proteins). Remarkably, the native E. coli maturation machinery will also generate a functional hydrogenase when provided with only the genes encoding the hydrogenase subunits and a single protease from P. furiosus. Another novel feature is that their expression was induced by anaerobic conditions, whereby E. coli was grown aerobically and production of recombinant hydrogenase was achieved by simply changing the gas feed from air to an inert gas (N2). The recombinant enzyme was purified and shown to be functionally similar to the native enzyme purified from P. furiosus. The methodology to generate this key hydrogen-producing enzyme has dramatic implications for the production of hydrogen and NADPH as vehicles for energy storage and transport, for engineering hydrogenase to optimize production and catalysis, as well as for the general production of complex, oxygen-sensitive metalloproteins.
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