ArticlePLoS genetics2010
DNA adenine methylation is required to replicate both Vibrio cholerae chromosomes once per cell cycle.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 65 citations in OpenAlex.
- Genome replication and cell division control by the methylation-sensitive GcrA regulator in Agrobacterium tumefaciens.Nucleic acids research · 2026Article
- Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae.Nature communications · 2025Article
- The replication enhancer crtS depends on transcription factor Lrp for modulating binding of initiator RctB to ori2 of Vibrio cholerae.Nucleic acids research · 2024Article
- Cell cycle-coordinated maintenance of theEcoSal Plus · 2023Review
- Diverse Roles for a Conserved DNA-Methyltransferase in the Entomopathogenic BacteriumInternational journal of molecular sciences · 2022Article
- The coordinated replication of Vibrio cholerae's two chromosomes required the acquisition of a unique domain by the RctB initiator.Nucleic acids research · 2021Article
- Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2.Nucleic acids research · 2020Article
- Article
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
- Vibrio cholerae chromosome 2 copy number is controlled by the methylation-independent binding of its monomeric initiator to the chromosome 1 crtS site.Nucleic acids research · 2018Article
- Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Single Circular Chromosome Identified from the Genome Sequence of the Vibrio cholerae O1 bv. El Tor Ogawa Strain V060002.Genome announcements · 2018Article
- Chromosome 1 licenses chromosome 2 replication in Vibrio cholerae by doubling the crtS gene dosage.PLoS genetics · 2018Article
- Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae.PLoS genetics · 2018Article
- Functionality of Two Origins of Replication inFrontiers in microbiology · 2018Article
- Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes.Frontiers in microbiology · 2018Review
- Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli.Antibiotics (Basel, Switzerland) · 2017Article
- The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators.Nucleic acids research · 2017Article
- Article
- Random versus Cell Cycle-Regulated Replication Initiation in Bacteria: Insights from Studying Vibrio cholerae Chromosome 2.Microbiology and molecular biology reviews : MMBR · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
DNA adenine methylation is widely used to control many DNA transactions, including replication. In Escherichia coli, methylation serves to silence newly synthesized (hemimethylated) sister origins. SeqA, a protein that binds to hemimethylated DNA, mediates the silencing, and this is necessary to restrict replication to once per cell cycle. The methylation, however, is not essential for replication initiation per se but appeared so when the origins (oriI and oriII) of the two Vibrio cholerae chromosomes were used to drive plasmid replication in E. coli. Here we show that, as in the case of E. coli, methylation is not essential for oriI when it drives chromosomal replication and is needed for once-per-cell-cycle replication in a SeqA-dependent fashion. We found that oriII also needs SeqA for once-per-cell-cycle replication and, additionally, full methylation for efficient initiator binding. The requirement for initiator binding might suffice to make methylation an essential function in V. cholerae. The structure of oriII suggests that it originated from a plasmid, but unlike plasmids, oriII makes use of methylation for once-per-cell-cycle replication, the norm for chromosomal but not plasmid replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.